pharmjournalРазработка и регистрация лекарственных средствDrug development & registration2305-20662658-5049LLC «CPHA»10.33380/2305-2066-2021-10-4-46-53pharmjournal-1065Research ArticleПОИСК И РАЗРАБОТКА НОВЫХ ЛЕКАРСТВЕННЫХ СРЕДСТВRESEARCH AND DEVELOPMENT OF NEW DRUG PRODUCTSАнтиоксидантная активность серебросодержащих бионанокомпозиций на основе гуминовых веществ в культуре клетокAntioxidant Activity of Silver-containing Bionanocompositions Based on Humic Substances in Cell Culturehttps://orcid.org/0000-0002-6714-1938БуйкоЕ. Е.BuykoE. E.

Буйко Евгений Евгеньевич

634050, Томская обл., Томск, Московский тракт, д. 2; 634034, Томск, пр. Ленина, д. 30

Evgeny E. Buyko

2, Moskovsky tract, Tomsk, Tomsk region, 634050; 30, Lenina ave., Tomsk, 634034

buykoevgen@yandex.ruhttps://orcid.org/0000-0002-1973-8983ЗыковаМ. В.ZykovaM. V.

634050, Томская обл., Томск, Московский тракт, д. 2

Maria V. Zykova

2, Moskovsky tract, Tomsk, Tomsk region, 634050

https://orcid.org/0000-0001-9348-4945ИвановВ. В.IvanovV. V.

634050, Томская обл., Томск, Московский тракт, д. 2

Vladimir V. Ivanov

2, Moskovsky tract, Tomsk, Tomsk region, 634050

https://orcid.org/0000-0001-6571-4061БратишкоК. А.BratishkoK. A.

634050, Томская обл., Томск, Московский тракт, д. 2; 634034, Томск, пр. Ленина, д. 30

Kristina A. Bratishko

2, Moskovsky tract, Tomsk, Tomsk region, 634050; 30, Lenina ave., Tomsk, 634034

https://orcid.org/0000-0002-3837-1179УфандеевА. А.UfandeevA. A.

634050, Томская обл., Томск, Московский тракт, д. 2

Alexander A. Ufandeev

2, Moskovsky tract, Tomsk, Tomsk region, 634050

https://orcid.org/0000-0002-7978-9774ГригорьеваИ. О.GrigorievaI. O.

119991, Москва, Ленинские горы, д. 1

Irina O. Grigorieva

1, Leninskie gory, Moscow, 119991

https://orcid.org/0000-0001-7169-8846ЦупкоА. В.TsupkoA. V.

634050, Томская обл., Томск, Московский тракт, д. 2

Andrey V. Tsupko

2, Moskovsky tract, Tomsk, Tomsk region, 634050

https://orcid.org/0000-0002-5292-1368МихалёвД. А.MikhalyovD. A.

634050, Томская обл., Томск, Московский тракт, д. 2

Dmitry A. Mikhalyov

2, Moskovsky tract, Tomsk, Tomsk region, 634050

https://orcid.org/0000-0001-9084-7851ПерминоваИ. В.PerminovaI. V.

119991, Москва, Ленинские горы, д. 1

Irina V. Perminova

1, Leninskie gory, Moscow, 119991

https://orcid.org/0000-0002-2153-7945БелоусовМ. В.BelousovM. V.

634050, Томская обл., Томск, Московский тракт, д. 2; 634034, Томск, пр. Ленина, д. 30

Mikhail V. Belousov

2, Moskovsky tract, Tomsk, Tomsk region, 634050; 30, Lenina ave., Tomsk, 634034

ФГБОУ ВО Сибирский государственный медицинский университет Министерства здравоохранения Российской Федерации (ФГБОУ ВО СибГМУ Минздрава России); ФГАОУ ВО Национальный исследовательский Томский политехнический университет (ТПУ)Siberian State Medical University, SSMU; The National Research Tomsk Polytechnic UniversityФГБОУ ВО Сибирский государственный медицинский университет Министерства здравоохранения Российской Федерации (ФГБОУ ВО СибГМУ Минздрава России)Siberian State Medical University, SSMUФГБОУ ВО Московский государственный университет имени М. В. Ломоносова (МГУ имени М. В. Ломоносова)Lomonosov Moscow State University2021231120211044653Copyright © Буйко Е.Е., Зыкова М.В., Иванов В.В., Братишко К.А., Уфандеев А.А., Григорьева И.О., Цупко А.В., Михалёв Д.А., Перминова И.В., Белоусов М.В., 20212021Буйко Е.Е., Зыкова М.В., Иванов В.В., Братишко К.А., Уфандеев А.А., Григорьева И.О., Цупко А.В., Михалёв Д.А., Перминова И.В., Белоусов М.В.Buyko E.E., Zykova M.V., Ivanov V.V., Bratishko K.A., Ufandeev A.A., Grigorieva I.O., Tsupko A.V., Mikhalyov D.A., Perminova I.V., Belousov M.V.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.pharmjournal.ru/jour/article/view/1065Введение

Введение. Наночастицы серебра являются перспективными агентами для подавления резистентных штаммов микроорганизмов и ускорения заживления гнойных ран. Окислительный стресс нарушает процессы нормального ранозаживления, что приводит к образованию хронических незаживающих ран. Поэтому определение способности новых ранозаживляющих агентов подавлять продукцию активных форм кислорода является актуальной задачей.

Цель

Цель. Исследовать влияние серебросодержащих бионанокомпозиций на основе гуминовых веществ на базальную и стимулированную трет-бутилгидропероксидом продукцию активных форм кислорода в культуре клеток нормальных фибробластов 3T3-L1 in vitro.

Материалы и методы

Материалы и методы. Исследование проведено на 7 образцах исходных гуминовых веществ и соответствующих им биоматериалах с наночастицами серебра, синтезированных в Лаборатории природных гуминовых систем химического факультета Московского государственного университета имени М. В. Ломоносова. Оценка внутриклеточной продукции активных форм кислорода проводилась с использованием флуоресцентного зонда 2,7-дихлородигидрофлуоресцеиндиацетата. Клетки культивировали с образцами в течение 24 ч, для стимуляции продукции активных форм кислорода использовали трет-бутилгидропероксид. Детекцию осуществляли флуориметрически с помощью микропланшетного ридера.

Результаты и обсуждение

Результаты и обсуждение. Наиболее выраженную антиоксидантную активность продемонстрировали три образца биоматериалов с наночастицами серебра, ультрадиспергированными в матрицах гуминовых веществ (CHS-AgNPs, CHP-AgNPs и CHE-AgNPs), что позволяет рассматривать их как наиболее перспективные в качестве фармацевтических агентов для лечения гнойно-воспалительных процессов. Наиболее вероятным механизмом высокой антиоксидантной активности исследуемых биоматериалов в отношении интрацеллюлярных активных форм кислорода является собственная активность гуминовых веществ по связыванию активных форм кислорода, в то время как наночастицы серебра в составе биоматериалов катализируют восстановительные процессы их взаимодействия с активными формами кислорода.

Заключение

Заключение. Для исследуемых образцов биоматериалов с наночастицами серебра, ультрадиспергированными в матрицах гуминовых веществ, характерна выраженная антиоксидантная активность, что вместе с антибактериальными свойствами позволяет рассматривать их как потенциальные агенты для ускорения заживления гнойных ран.

Introduction

Introduction. Silver nanoparticles are promising agents for suppressing resistant strains of microorganisms and accelerating the purulent wounds healing. Oxidative stress disrupts normal wound healing processes, which leads to the formation of chronic non-healing wounds. Therefore, the determination of the ability of new wound healing agents to decrease the production of reactive oxygen species is a relevant task.

Aim

Aim. The aim of the current study was to investigate the effect of silver-containing bionanocompositions based on humic substances on the basal and tert-butyl hydroperoxide-stimulated production of reactive oxygen species at the normal fibroblasts 3T3-L1 cell culture in vitro.

Materials and methods

Materials and methods. The study was carried out on 7 samples of initial humic substances and biomaterials with silver nanoparticles synthesized in the Laboratory of Natural Humic Systems, Faculty of Chemistry, Moscow State University named after M. V. Lomonosov. The intracellular production of reactive oxygen species was assessed using a 2,7-dichlorodihydrofluorescein diacetate fluorescent probe. Cells were cultured with samples for 24 h; tret-butyl hydroperoxide was used to stimulate the production of reactive oxygen species. Detection was performed fluorometrically using a microplate reader.

Results and discussion

Results and discussion. The most pronounced antioxidant activity was demonstrated by three samples of biomaterials with silver nanoparticles ultradispersed in humic substances matrices (CHS-AgNPs, CHP-AgNPs and CHE-AgNPs), which allows us to consider them as the most promising pharmaceutical agents for the treatment of purulent-inflammatory processes. The most probable mechanism of the high antioxidant activity of the studied biomaterials in relation to intracellular reactive oxygen species is the intrinsic activity of humic substances to bind reactive oxygen species, while silver nanoparticles in biomaterials catalyze the reduction processes of their interaction with reactive oxygen species.

Conclusion

Conclusion. For the studied samples of biomaterials with silver nanoparticles ultradispersed in matrices of humic substances pronounced antioxidant activity was shown. Together with antibacterial properties, it makes it possible to consider them as potential agents for purulent wounds healing accelerating.

гуминовые веществананочастицы серебраантиоксидантная активностьтрет-бутилгидропероксидранозаживлениеhumic substancessilver nanoparticlesantioxidant activitytert-butyl hydroperoxidewound healingНастоящее исследование выполнено при финансовой поддержке Российского Научного Фонда (грант № 20-65-47052)This research was carried out with the financial support of the Russian Science Foundation (Grant No. 20-65-47052)ReferencesAnjum S., Hashim M., Malik S. A., Khan M., Lorenzo J. M., Abbasi B. H., Hano, C. Recent Advances in Zinc Oxide Nanoparticles (ZnO NPs) for Cancer Diagnosis, Target Drug Delivery, and Treatment. Cancers. 2021;13(18):4570. DOI: 10.3390/cancers13184570.Anjum S., Hashim M., Malik S. A., Khan M., Lorenzo J. M., Abbasi B. H., Hano, C. Recent Advances in Zinc Oxide Nanoparticles (ZnO NPs) for Cancer Diagnosis, Target Drug Delivery, and Treatment. Cancers. 2021;13(18):4570. DOI: 10.3390/cancers13184570.Abbasi E., Milani M., Fekri Aval S., Kouhi M., Akbarzadeh A., Tayefi Nasrabadi H., Nikasa P., Joo S.W., Hanifehpour Y., Nejati-Koshki K., Samiei M. Silver nanoparticles: synthesis methods, bio-applications and properties. Critical reviews in microbiology. 2016;42(2):173-180. DOI: 10.3109/1040841X.2014.912200.Abbasi E., Milani M., Fekri Aval S., Kouhi M., Akbarzadeh A., Tayefi Nasrabadi H., Nikasa P., Joo S.W., Hanifehpour Y., Nejati-Koshki K., Samiei M. Silver nanoparticles: synthesis methods, bio-applications and properties. Critical reviews in microbiology. 2016;42(2):173-180. DOI: 10.3109/1040841X.2014.912200.Paladini F., Pollini M. Antimicrobial silver nanoparticles for wound healing application: progress and future trends. Materials. 2019;12(16):2540. DOI: 10.3390/ma12162540.Paladini F., Pollini M. Antimicrobial silver nanoparticles for wound healing application: progress and future trends. Materials. 2019;12(16):2540. DOI: 10.3390/ma12162540.GhavamiNejad A., Park C. H., Kim C. S. In situ synthesis of antimicrobial silver nanoparticles within antifouling zwitterionic hydrogels by catecholic redox chemistry for wound healing application. Biomacromolecules. 2016;17(3):1213-1223. DOI: 10.1021/acs.biomac.6b00039.GhavamiNejad A., Park C. H., Kim C. S. In situ synthesis of antimicrobial silver nanoparticles within antifouling zwitterionic hydrogels by catecholic redox chemistry for wound healing application. Biomacromolecules. 2016;17(3):1213-1223. DOI: 10.1021/acs.biomac.6b00039.Tian X., Jiang X., Welch C., Croley T. R., Wong T. Y., Chen C., Fan S., Chong Y., Li R., Ge C., Chen C., Yin J. J. Bactericidal effects of silver nanoparticles on lactobacilli and the underlying mechanism. ACS applied materials & interfaces. 2018;10(10):8443-8450. DOI: 10.1021/acsami.7b17274.Tian X., Jiang X., Welch C., Croley T. R., Wong T. Y., Chen C., Fan S., Chong Y., Li R., Ge C., Chen C., Yin J. J. Bactericidal effects of silver nanoparticles on lactobacilli and the underlying mechanism. ACS applied materials & interfaces. 2018;10(10):8443-8450. DOI: 10.1021/acsami.7b17274.Netala V. R., Bethu M. S., Pushpalatha B., Baki V. B., Aishwarya S., Rao J. V., Tartte V. Biogenesis of silver nanoparticles using endophytic fungus Pestalotiopsis microspora and evaluation of their antioxidant and anticancer activities. International journal of nanomedicine. 2016;11:5683. DOI: 10.2147/IJN.S112857.Netala V. R., Bethu M. S., Pushpalatha B., Baki V. B., Aishwarya S., Rao J. V., Tartte V. Biogenesis of silver nanoparticles using endophytic fungus Pestalotiopsis microspora and evaluation of their antioxidant and anticancer activities. International journal of nanomedicine. 2016;11:5683. DOI: 10.2147/IJN.S112857.Comino-Sanz I. M., Lopez-Franco M. D., Castro B., Pancorbo-Hidalgo P. L. The Role of Antioxidants on Wound Healing: A Review of the Current Evidence. Journal of Clinical Medicine. 2021;10(16):3558. DOI: 10.3390/jcm10163558.Comino-Sanz I. M., Lopez-Franco M. D., Castro B., Pancorbo-Hidalgo P. L. The Role of Antioxidants on Wound Healing: A Review of the Current Evidence. Journal of Clinical Medicine. 2021;10(16):3558. DOI: 10.3390/jcm10163558.Velnar T., Bailey T., Smrkolj V. The wound healing process: an overview of the cellular and molecular mechanisms. Journal of International Medical Research. 2009;37(5):1528-1542. DOI: 10.1177/147323000903700531.Velnar T., Bailey T., Smrkolj V. The wound healing process: an overview of the cellular and molecular mechanisms. Journal of International Medical Research. 2009;37(5):1528-1542. DOI: 10.1177/147323000903700531.Rosenbaum A. J., Banerjee S., Rezak K. M., Uhl R. L. Advances in wound management. Journal of the American Academy of Orthopaedic Surgeons. 2018;26(23):833-843. DOI: 10.5435/JAAOS-D-17-00024.Rosenbaum A. J., Banerjee S., Rezak K. M., Uhl R. L. Advances in wound management. Journal of the American Academy of Orthopaedic Surgeons. 2018;26(23):833-843. DOI: 10.5435/JAAOS-D-17-00024.Cano Sanchez M., Lancel S., Boulanger E., Neviere R. Targeting oxidative stress and mitochondrial dysfunction in the treatment of impaired wound healing: a systematic review. Antioxidants (Basel). 2018;7(8):98. DOI: 10.3390/antiox7080098.Cano Sanchez M., Lancel S., Boulanger E., Neviere R. Targeting oxidative stress and mitochondrial dysfunction in the treatment of impaired wound healing: a systematic review. Antioxidants (Basel). 2018;7(8):98. DOI: 10.3390/antiox7080098.Rodriguez P. G., Felix F. N., Woodley D. T., Shim E. K. The role of oxygen in wound healing: a review of the literature. Dermatologic surgery. 2008;34(9):1159-1169. DOI: 10.1177/147323000903700531.Rodriguez P. G., Felix F. N., Woodley D. T., Shim E. K. The role of oxygen in wound healing: a review of the literature. Dermatologic surgery. 2008;34(9):1159-1169. DOI: 10.1177/147323000903700531.Bryan N., Ahswin H., Smart N., Bayon Y., Wohlert S., Hunt J. A. Reactive oxygen species (ROS)-a family of fate deciding molecules pivotal in constructive inflammation and wound healing. Eur Cell Mater. 2012;24:249-265. DOI: 10.22203/ecm.v024a18.Bryan N., Ahswin H., Smart N., Bayon Y., Wohlert S., Hunt J. A. Reactive oxygen species (ROS)-a family of fate deciding molecules pivotal in constructive inflammation and wound healing. Eur Cell Mater. 2012;24:249-265. DOI: 10.22203/ecm.v024a18.Dunnill C., Patton T., Brennan J., Barrett J., Dryden M., Cooke J., Leaper D., Georgopoulos N. T. Reactive oxygen species (ROS) and wound healing: the functional role of ROS and emerging ROS-modulating technologies for augmentation of the healing process. International wound journal. 2017;14(1):89-96. DOI: 10.1111/iwj.12557.Dunnill C., Patton T., Brennan J., Barrett J., Dryden M., Cooke J., Leaper D., Georgopoulos N. T. Reactive oxygen species (ROS) and wound healing: the functional role of ROS and emerging ROS-modulating technologies for augmentation of the healing process. International wound journal. 2017;14(1):89-96. DOI: 10.1111/iwj.12557.Musino D., Devcic J., Lelong C., Luche S., Rivard C., Dalzon B., Land-rot G., Rabilloud T., Capron, I. Impact of Physico-Chemical Properties of Cellulose Nanocrystal/Silver Nanoparticle Hybrid Suspensions on Their Biocidal and Toxicological Effects. Nanomaterials. 2021;11(7):1862. DOI: 10.3390/nano11071862.Musino D., Devcic J., Lelong C., Luche S., Rivard C., Dalzon B., Land-rot G., Rabilloud T., Capron, I. Impact of Physico-Chemical Properties of Cellulose Nanocrystal/Silver Nanoparticle Hybrid Suspensions on Their Biocidal and Toxicological Effects. Nanomaterials. 2021;11(7):1862. DOI: 10.3390/nano11071862.Alahmad A., Feldhoff A., Bigall N. C., Rusch P., Scheper T., Walter J. G. Hypericum perforatum L.-mediated green synthesis of silver nanoparticles exhibiting antioxidant and anticancer activities. Nanomaterials. 2021;11(2);487. DOI: 10.3390/nano11020487.Alahmad A., Feldhoff A., Bigall N. C., Rusch P., Scheper T., Walter J. G. Hypericum perforatum L.-mediated green synthesis of silver nanoparticles exhibiting antioxidant and anticancer activities. Nanomaterials. 2021;11(2);487. DOI: 10.3390/nano11020487.Nguyen T. H. A., Nguyen V. C., Phan T. N. H., Le V. T., Vasseghian Y., Trubitsyn M. A., Nguyen A. T., Chau T. P., Doan V. D. Novel biogenic silver and gold nanoparticles for multifunctional applications: Green synthesis, catalytic and antibacterial activity, and colorimetric detection of Fe (III) ions. Chemosphere. 2021;287(3):132271. DOI: 10.1016/j.chemosphere.2021.132271.Nguyen T. H. A., Nguyen V. C., Phan T. N. H., Le V. T., Vasseghian Y., Trubitsyn M. A., Nguyen A. T., Chau T. P., Doan V. D. Novel biogenic silver and gold nanoparticles for multifunctional applications: Green synthesis, catalytic and antibacterial activity, and colorimetric detection of Fe (III) ions. Chemosphere. 2021;287(3):132271. DOI: 10.1016/j.chemosphere.2021.132271.Aboyewa J. A., Sibuyi N. R., Meyer M., Oguntibeju O. O. Green Synthesis of Metallic Nanoparticles Using Some Selected Medicinal Plants from Southern Africa and Their Biological Applications. Plants. 2021;10(9);1929. DOI: 10.3390/plants10091929.Aboyewa J. A., Sibuyi N. R., Meyer M., Oguntibeju O. O. Green Synthesis of Metallic Nanoparticles Using Some Selected Medicinal Plants from Southern Africa and Their Biological Applications. Plants. 2021;10(9);1929. DOI: 10.3390/plants10091929.Zykova M. V., Schepetkin I. A., Belousov M. V., Krivoshchekov S. V., Logvinova L. A., Bratishko K. A., Yusubov M. S., Romanenko S. V., Quinn M. T. Physicochemical characterization and antioxidant activity of humic acids isolated from peat of various origins. Molecules. 2018;23(4):753. DOI: 10.3390/molecules23040753.Zykova M. V., Schepetkin I. A., Belousov M. V., Krivoshchekov S. V., Logvinova L. A., Bratishko K. A., Yusubov M. S., Romanenko S. V., Quinn M. T. Physicochemical characterization and antioxidant activity of humic acids isolated from peat of various origins. Molecules. 2018;23(4):753. DOI: 10.3390/molecules23040753.Orlov D. S. Humus acids of soils. Rotterdam: AA Balkema; 1985. 378 p.Orlov D. S. Humus acids of soils. Rotterdam: AA Balkema; 1985. 378 p.Schepetkin I., Khlebnikov A., Kwon B. S. Medical drugs from humus matter: focus on mumie. Drug development research. 2002;57(3):140-159. DOI: 10.1002/ddr.10058.Schepetkin I., Khlebnikov A., Kwon B. S. Medical drugs from humus matter: focus on mumie. Drug development research. 2002;57(3):140-159. DOI: 10.1002/ddr.10058.Trofimova E. S., Zykova M. V., Ligacheva A. A., Sherstoboev E. Y., Zhdanov V. V., Belousov M. V., Krivoshchekov S. V., Danilets M. G., Dygai A. M. Influence of humic acids extracted from peat by different methods on functional activity of macrophages in vitro. Bulletin of experimental biology and medicine. 2017;162(6):741-745. DOI: 10.1007/s10517-017-3702-5.Trofimova E. S., Zykova M. V., Ligacheva A. A., Sherstoboev E. Y., Zhdanov V. V., Belousov M. V., Krivoshchekov S. V., Danilets M. G., Dygai A. M. Influence of humic acids extracted from peat by different methods on functional activity of macrophages in vitro. Bulletin of experimental biology and medicine. 2017;162(6):741-745. DOI: 10.1007/s10517-017-3702-5.Joone G. K., Dekker J., van Rensburg C. E. J. Investigation of the immunostimulatory properties of oxihumate. Zeitschrift fur Naturforschung. C, Journal of biosciences. 2003;58(3-4):263-267. DOI: 10.1515/znc-2003-3-421.Joone G. K., Dekker J., van Rensburg C. E. J. Investigation of the immunostimulatory properties of oxihumate. Zeitschrift fur Naturforschung. C, Journal of biosciences. 2003;58(3-4):263-267. DOI: 10.1515/znc-2003-3-421.Жаркова Л. П., Князева И. P, Иванов В. В., Большаков М. А., Кутенков О. П., Ростов В. В. Влияние импульсно-периодического рентгеновского и микроволнового излучений на уровень перекисей в изолированных гепатоцитах. Вестник Томского государственного университета. 2010;333:161-163.Zharkova L. P., Knyazeva I. R., Ivanov V. V., Bolshakov M. A., Kutenkov O. P., Rostov V. V. Influence of repetitively pulsed X-ray and microwave radiation on the level of peroxides in isolated hepatocytes. Bulletin of Tomsk State University. 2010;333:161-163. (In Russ.)Laghrib F., Houcini H., Khalil F., Liba A., Bakasse M., Lahrich S., El Mhammedi M. A. Synthesis of Silver Nanoparticles Using Chitosan as Stabilizer Agent: Application towards Electrocatalytical Reduction of p-Nitrophenol. ChemistrySelect. 2020;5(3):1220-1227. DOI: 10.1002/slct.201903955.Laghrib F., Houcini H., Khalil F., Liba A., Bakasse M., Lahrich S., El Mhammedi M. A. Synthesis of Silver Nanoparticles Using Chitosan as Stabilizer Agent: Application towards Electrocatalytical Reduction of p-Nitrophenol. ChemistrySelect. 2020;5(3):1220-1227. DOI: 10.1002/slct.201903955.Nasirizadeh N., Shekari Z., Dehghani M., Makarem S. Delphinidin immobilized on silver nanoparticles for the simultaneous determination of ascorbic acid, noradrenalin, uric acid, and tryptophan. Journal of food and drug analysis. 2016;24(2):406-416. DOI: 10.1016/j.jfda.2015.11.011.Nasirizadeh N., Shekari Z., Dehghani M., Makarem S. Delphinidin immobilized on silver nanoparticles for the simultaneous determination of ascorbic acid, noradrenalin, uric acid, and tryptophan. Journal of food and drug analysis. 2016;24(2):406-416. DOI: 10.1016/j.jfda.2015.11.011.Ha D. T., Oh J., Khoi N. M., Dao T. T., Dung L. V., Do T. N. Q., Lee S. M., Jang T. S., Jeong G., Na M. In vitro and in vivo hepatoprotective effect of ganodermanontriol against t-BHP-induced oxidative stress. Journal of ethnopharmacology. 2013;150(3):875-885. DOI: 10.1016/j.jep.2013.09.039.Ha D. T., Oh J., Khoi N. M., Dao T. T., Dung L. V., Do T. N. Q., Lee S. M., Jang T. S., Jeong G., Na M. In vitro and in vivo hepatoprotective effect of ganodermanontriol against t-BHP-induced oxidative stress. Journal of ethnopharmacology. 2013;150(3):875-885. DOI: 10.1016/j.jep.2013.09.039.Ye H., Luo J., Hu D., Yang S., Zhang A., Qiu Y., Ma X., Wang J., Hou J., Bai J. Total Flavonoids of Crocus sativus Petals Release tert-Butyl Hydroperoxide-Induced Oxidative Stress in BRL-3A Cells. Oxidative Medicine and Cellular Longevity. 2021;2021:1-15. DOI: 10.1155/2021/5453047.Ye H., Luo J., Hu D., Yang S., Zhang A., Qiu Y., Ma X., Wang J., Hou J., Bai J. Total Flavonoids of Crocus sativus Petals Release tert-Butyl Hydroperoxide-Induced Oxidative Stress in BRL-3A Cells. Oxidative Medicine and Cellular Longevity. 2021;2021:1-15. DOI: 10.1155/2021/5453047.Заводник И. Б. Образование активных форм кислорода и морфологические изменения клеток линии B14 при окислительном воздействии, индуцируемом органическим гидропероксидом. Известия Национальной академии наук Беларуси. Серия биологических наук. 2015;3:104-108.Zavodnik I. B. Reactive oxygen species generation and B14 cells morphological transformations under oxidative treatment by organic hydroperoxide. Izvestiya Natsional'noy akademii nauk Belaru-si. Seriya biologicheskikh nauk = Proceedings of the National Academy of Sciences of Belarus. Biological Series. 2015;3:104-108. (In Russ.)Bedlovicova Z., Strapac I., Balaz M., Salayova A. A brief overview on antioxidant activity determination of silver nanoparticles. Molecule. 2020;25(14):3191. DOI: 10.3390/molecules25143191.Bedlovicova Z., Strapac I., Balaz M., Salayova A. A brief overview on antioxidant activity determination of silver nanoparticles. Molecule. 2020;25(14):3191. DOI: 10.3390/molecules25143191.Aeschbacher M., Graf C., Schwarzenbach R. P., Sander M. Antioxidant properties of humic substances. Environ Sci Technol. 2012;1;46(9):4916-4925. DOI: 10.1021/es300039h.Aeschbacher M., Graf C., Schwarzenbach R. P., Sander M. Antioxidant properties of humic substances. Environ Sci Technol. 2012;1;46(9):4916-4925. DOI: 10.1021/es300039h.Братишко К. А., Зыкова М. В., Иванов В. В., Буйко Е. Е., Дрыгунова Л. А., Перминова И. В., Белоусов М. В. Гуминовые кислоты торфа - перспективные биологически активные вещества с антиоксидантной активностью для разработки протекторных средств. Химия растительного сырья. 2021;1:287-298. DOI: 10.14258/jcprm.2021018784Bratishko K. A., Zykova M. V., Ivanov V. V., Buyko E. E., Drygunova L. A., Perminova I. V., Belousov M. V. Peat humic acids-prospective biologically active substances with antioxidant activity for the development of protective agents. Khimiya Rastitel'nogo Syr'ya. 2021;1:287-298. (In Russ.) DOI: 10.14258/jcprm.2021018784.Klavins M., Sire J. Variations of humic acids properties within peat profiles. Mires and peat. 2010;4:175-197.Klavins M., Sire J. Variations of humic acids properties within peat profiles. Mires and peat. 2010;4:175-197.Vijilvani C., Bindhu M. R., Frincy F. C., AlSalhi M. S., Sabitha S., Saravanakumar K., Devanesan S., Umavedi M., Aljaafreh M. J., Atif M. Antimicrobial and catalytic activities of biosynthesized gold, silver and palladium nanoparticles from Solanum nigurum leaves. Journal of Photochemistry and Photobiology B: Biology. 2020;202:111713. DOI: 10.1016/j.jphotobiol.2019.111713.Vijilvani C., Bindhu M. R., Frincy F. C., AlSalhi M. S., Sabitha S., Saravanakumar K., Devanesan S., Umavedi M., Aljaafreh M. J., Atif M. Antimicrobial and catalytic activities of biosynthesized gold, silver and palladium nanoparticles from Solanum nigurum leaves. Journal of Photochemistry and Photobiology B: Biology. 2020;202:111713. DOI: 10.1016/j.jphotobiol.2019.111713.Du L., Xu Q., Huang M., Xian L., Feng J. X. Synthesis of small silver nanoparticles under light radiation by fungus Penicillium oxalicum and its application for the catalytic reduction of methylene blue. Materials Chemistry and Physics. 2015;160:40-47. DOI: 10.1016/j.matchemphys.2015.04.003.Du L., Xu Q., Huang M., Xian L., Feng J. X. Synthesis of small silver nanoparticles under light radiation by fungus Penicillium oxalicum and its application for the catalytic reduction of methylene blue. Materials Chemistry and Physics. 2015;160:40-47. DOI: 10.1016/j.matchemphys.2015.04.003.Hu D., Yang X., Chen W., Feng Z., Hu C., Yan F., Chen X., Qu D., Chen Z. Rhodiola rosea Rhizome Extract-Mediated Green Synthesis of Silver Nanoparticles and Evaluation of Their Potential Antioxidant and Catalytic Reduction Activities. ACS omega. 2021;6(38):24450-24461. DOI: 10.1021/acsomega.1c02843.Hu D., Yang X., Chen W., Feng Z., Hu C., Yan F., Chen X., Qu D., Chen Z. Rhodiola rosea Rhizome Extract-Mediated Green Synthesis of Silver Nanoparticles and Evaluation of Their Potential Antioxidant and Catalytic Reduction Activities. ACS omega. 2021;6(38):24450-24461. DOI: 10.1021/acsomega.1c02843.Bharadwaj K. K., Rabha B., Pati S., Choudhury B. K., Sarkar T., Gogoi S. K., Kakati N., Baishya D., Kari Z. A., Edinur H. A. Green Synthesis of Silver Nanoparticles Using Diospyros malabarica Fruit Extract and Assessments of Their Antimicrobial, Anticancer and Catalytic Reduction of 4-Nitrophenol (4-NP). Nanomaterials. 2021;11(8):1999. DOI: 10.1021/acsomega.1c02843.Bharadwaj K. K., Rabha B., Pati S., Choudhury B. K., Sarkar T., Gogoi S. K., Kakati N., Baishya D., Kari Z. A., Edinur H. A. Green Synthesis of Silver Nanoparticles Using Diospyros malabarica Fruit Extract and Assessments of Their Antimicrobial, Anticancer and Catalytic Reduction of 4-Nitrophenol (4-NP). Nanomaterials. 2021;11(8):1999. DOI: 10.1021/acsomega.1c02843.

The authors declare that there are no conflicts of interest present.