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<article article-type="research-article" dtd-version="1.3" xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xml:lang="ru"><front><journal-meta><journal-id journal-id-type="publisher-id">pharmjournal</journal-id><journal-title-group><journal-title xml:lang="ru">Разработка и регистрация лекарственных средств</journal-title><trans-title-group xml:lang="en"><trans-title>Drug development &amp; registration</trans-title></trans-title-group></journal-title-group><issn pub-type="ppub">2305-2066</issn><issn pub-type="epub">2658-5049</issn><publisher><publisher-name>LLC «CPHA»</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="doi">10.33380/2305-2066-2021-10-4-138-146</article-id><article-id custom-type="elpub" pub-id-type="custom">pharmjournal-1074</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="ru"><subject>МЕТОДЫ АНАЛИЗА ЛЕКАРСТВЕННЫХ СРЕДСТВ</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="en"><subject>ANALYTICAL METHODS</subject></subj-group></article-categories><title-group><article-title>Количественные анатомические характеристики листовых пластинок некоторых видов Crataegus L.</article-title><trans-title-group xml:lang="en"><trans-title>Quantitative Anatomical Characteristics of the Leaf Blades of the Several Species of Crataegus L.</trans-title></trans-title-group></title-group><contrib-group><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0001-5526-7675</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Сагарадзе</surname><given-names>В. А.</given-names></name><name name-style="western" xml:lang="en"><surname>Sagaradze</surname><given-names>V. A.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Сагарадзе Валентина Андреевна</p><p>117216, Москва, улица Грина, д. 7</p></bio><bio xml:lang="en"><p>Valentina A. Sagaradze</p><p>7/1, Greena str., Moscow, 117216</p></bio><email xlink:type="simple">valentina.sagaradze@yandex.ru</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0002-4992-6926</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Бабаева</surname><given-names>Е. Ю.</given-names></name><name name-style="western" xml:lang="en"><surname>Babaeva</surname><given-names>E. Yu.</given-names></name></name-alternatives><bio xml:lang="ru"><p>117216, Москва, улица Грина, д. 7</p></bio><bio xml:lang="en"><p>Elena Yu. Babaeva7/1, Greena str., Moscow, 117216</p></bio><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0003-0068-2788</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Каленикова</surname><given-names>Е. И.</given-names></name><name name-style="western" xml:lang="en"><surname>Kalenikova</surname><given-names>E. I.</given-names></name></name-alternatives><bio xml:lang="ru"><p>факультет фундаментальной медицины</p><p>119192, Москва, Ломоносовский пр., д. 27, корп. 1</p></bio><bio xml:lang="en"><p>Elena I. Kalenikova - Faculty of Fundamental Medicine.</p><p>27/1, Lomonosovskiy av., Moscow, 119192</p></bio><xref ref-type="aff" rid="aff-2"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0002-5147-6602</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Трусов</surname><given-names>Н. А.</given-names></name><name name-style="western" xml:lang="en"><surname>Trusov</surname><given-names>N. A.</given-names></name></name-alternatives><bio xml:lang="ru"><p>127276, Москва, Ботаническая ул., д. 4</p></bio><bio xml:lang="en"><p>Nikolay A. Trusov</p><p>4, Botanicheskaya str., Moscow, 127276</p></bio><xref ref-type="aff" rid="aff-3"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0001-8299-7462</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Пещанская</surname><given-names>Е. В.</given-names></name><name name-style="western" xml:lang="en"><surname>Peshchanskaya</surname><given-names>E. V.</given-names></name></name-alternatives><bio xml:lang="ru"><p>355028, Ставропольский край, Ставрополь, ул. Ленина, д. 478</p></bio><bio xml:lang="en"><p>Ekaterina V. Peshchanskaya</p><p>478, Lenina str., Stavropol, Stavropol Territory, 355028</p></bio><xref ref-type="aff" rid="aff-4"/></contrib></contrib-group><aff-alternatives id="aff-1"><aff xml:lang="ru"><institution>ФГБНУ Всероссийский научно-исследовательский институт лекарственных и ароматических растений (ВИЛАР)</institution></aff><aff xml:lang="en"><institution>All-Russian Scientific Research Institute of Medicinal and Aromatic Plants</institution></aff></aff-alternatives><aff-alternatives id="aff-2"><aff xml:lang="ru"><institution>ФГБОУ ВО Московский государственный университет имени М. В. Ломоносова (МГУ имени М. В. Ломоносова)</institution></aff><aff xml:lang="en"><institution>Lomonosov Moscow State University</institution></aff></aff-alternatives><aff-alternatives id="aff-3"><aff xml:lang="ru"><institution>ФГБУН Главный ботанический сад им. Н.В. Цицина, РАН</institution></aff><aff xml:lang="en"><institution>Tsytsin Main Botanical Garden, Russian Academy of Sciences</institution></aff></aff-alternatives><aff-alternatives id="aff-4"><aff xml:lang="ru"><institution>ФГБНУ Ставропольский Ботанический сад имени В.В. Скрипчинского</institution></aff><aff xml:lang="en"><institution>Skripchinsky Stavropol Botanical Garden</institution></aff></aff-alternatives><pub-date pub-type="collection"><year>2021</year></pub-date><pub-date pub-type="epub"><day>25</day><month>11</month><year>2021</year></pub-date><volume>10</volume><issue>4</issue><fpage>138</fpage><lpage>146</lpage><permissions><copyright-statement>Copyright &amp;#x00A9; Сагарадзе В.А., Бабаева Е.Ю., Каленикова Е.И., Трусов Н.А., Пещанская Е.В., 2021</copyright-statement><copyright-year>2021</copyright-year><copyright-holder xml:lang="ru">Сагарадзе В.А., Бабаева Е.Ю., Каленикова Е.И., Трусов Н.А., Пещанская Е.В.</copyright-holder><copyright-holder xml:lang="en">Sagaradze V.A., Babaeva E.Y., Kalenikova E.I., Trusov N.A., Peshchanskaya E.V.</copyright-holder><license xml:lang="ru" license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>Данная работа распространяется под лицензией Creative Commons Attribution 4.0.</license-p></license><license xml:lang="en" license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>This work is licensed under a Creative Commons Attribution 4.0 License.</license-p></license></permissions><self-uri xlink:href="https://www.pharmjournal.ru/jour/article/view/1074">https://www.pharmjournal.ru/jour/article/view/1074</self-uri><abstract><sec><title>Введение</title><p>Введение. Сырье растений рода Crataegus L. (Боярышник) включено во многие Фармакопеи мира. Государственная фармакопея Российской Федерации предусматривает использование плодов и цветков боярышника. Вместе с тем, литературные данные подтверждают возможность использования в медицинских целях листьев боярышника, поскольку «листья» и «цветки с листьями» имеют фармакопейный статус во всем мире. Они рассматриваются как перспективные виды сырья боярышника для российского фармацевтического производства. Однако большинство видов остаются плохо изученными в отношении количественных микроскопических характеристик (размеры устьичного аппарата (УА) и трихом эпидермиса листовых пластинок), которые могут иметь большое значение для установления подлинности сырья.</p></sec><sec><title>Цель</title><p>Цель. Изучить анатомическое строение эпидермиса листовых пластинок Crataegus spp. и провести сравнительное исследование количественных диагностических признаков листовых пластинок растений боярышника из секции Sanguineae и секции Crataegus, произрастающих в разных регионах Российской Федерации.</p></sec><sec><title>Материалы и методы</title><p>Материалы и методы. Образцы листьев боярышника (C. sanguinea, C. maximowiczii, C. dahurica, C. rhipidophylla, C. monogyna и C. pallasii) были заготовлены в естественных местообитаниях в Западной Сибири (Кемерово) и в дендрариях ботанических садов г. Москвы и г. Ставрополя. Измерения анатомических структур проводились с помощью светового микроскопа с окулярным микрометром.</p></sec><sec><title>Результаты и обсуждение</title><p>Результаты и обсуждение. Проведено сравнительное исследование морфолого-анатомического строения листовых пластинок (ЛП) на уровне видов и секции боярышника. Приведены размеры (длина и ширина) для УА, сидячих многоклеточных железок и простых одноклеточных трихом. Описана форма железок и установлено различие на уровне секции. Отмечены особенности опушения.</p></sec><sec><title>Заключение</title><p>Заключение. Результаты количественного анатомического исследования позволили выявить характерные отличительные признаки по размерам УА и форме желёзок в зависимости от вида боярышника и на уровне секций. Таким образом, это может облегчить установление подлинности и контроль качества целого или измельченного лекарственного сырья Crataegus.</p></sec></abstract><trans-abstract xml:lang="en"><sec><title>Introduction</title><p>Introduction. The Crataegus L. (Hawthorn) is a common herb in numerous Pharmacopoeias. The State Pharmacopoeia of the Russian Federation provides hawthorn fruits and flowers for medical utilization. With that, the literature data confirms the medical utility of hawthorn leaves since the “leaves” and the “flowers with leaves” have pharmacopoeial status worldwide. Therefore, those are considered as prospective forms of Crataegus raw material for Russian pharmaceutical production. However, most species remain poorly pharmacognostically investigated regarding the quantitative microscopic characteristics (the sizes of stomatal apparatus (SA) and epidermal leaf blade (LB) trichomes), which could be substantial for establishing the authenticity of the raw material.</p></sec><sec><title>Aim</title><p>Aim. Examine epidermal anatomy of Crataegus spp. Leaf blades (LBs) and perform a comparative study of several quantitative diagnostic features of LBs of hawthorn plants from the sect. Sanguineae and the sect. Crataegus, growing in diverse regions of the Russian Federation.</p></sec><sec><title>Materials and methods</title><p>Materials and methods. Samples of hawthorn leaves (C. sanguinea, C. maximowiczii, C. dahurica, C. rhipidophylla, C. monogyna and C. pallasii) were collected in natural habitats in Western Siberia (Kemerovo) and in arboretums of Botanical Gardens (Moscow, Stavropol). Measurements of anatomical structures were carried out using a light microscope accompanied by an ocular micrometre.</p></sec><sec><title>Results and discussion</title><p>Results and discussion. The LB surface phenotypic diversity within hawthorn species and sections was studied. The LBs were described in terms of meterages (longitude and width) of SA, meterages and shape of sedentary multicellular leaf teeth glands. The peculiarities of pubescence and the sizes of simple unicellular non-glandular trichomes were also observed.</p></sec><sec><title>Conclusion</title><p>Conclusion. The results of quantitative anatomical examination provided the characteristic features determining these elements at the species and section levels. Thus, it may facilitate authentication and quality control of whole or ground Crataegus medicinal raw material.</p></sec></trans-abstract><kwd-group xml:lang="ru"><kwd>виды Crataegus</kwd><kwd>листовая пластинка</kwd><kwd>желёзки на зубцах листа</kwd><kwd>устьичный аппарат</kwd><kwd>простые одноклеточные волоски</kwd></kwd-group><kwd-group xml:lang="en"><kwd>Crataegus species</kwd><kwd>leaf blade</kwd><kwd>leaf teeth glands</kwd><kwd>stomatal apparatus</kwd><kwd>simple unicellular trichomes</kwd></kwd-group><funding-group><funding-statement xml:lang="ru">Авторы выражают благодарность к. б. н. Р. А. Уфимову (Austrian Research and Training Centre for Forests, Natural Hazards and Landscapes, Vienna) за таксономическую идентификацию образцов; к. б. н. Ю. В. Загурской [Федеральный исследовательский центр угля и углехимии СО РАН (Институт экологии человека), Кемерово] за предоставленный растительный материал «Боярышника цветки с листьями». Работа поддержана Всероссийским научно-исследовательским институтом лекарственных и ароматических растений (ВИЛАР), Москва, Россия [Государственный бюджет НИОКР «Научное формирование, сохранение, изучение биоколлекций» № АААА-А19-119112590084-5 (0576-2019-0008)]</funding-statement><funding-statement xml:lang="en">We thank R. A. Ufimov, PhD (Austrian Research and Training Centre for Forests, Natural Hazards and Landscapes, Vienna) for the taxonomic identification of the raw material "Crataegi folium cum flore" specimens; Yu. V. Zagurskaya, PhD (Federal Research Center of Coal and Coal Chemistry SB RAS "Institute of Human Ecology", Kemerovo) for providing the raw material "Crataegi folium cum flore" samples. 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