Introduction

pharmjournal

Разработка и регистрация лекарственных средств

Drug development & registration

2305-20662658-5049

LLC «CPHA»

10.33380/2305-2066-2022-11-1-23-31

pharmjournal-1155

Research Article

ПОИСК И РАЗРАБОТКА НОВЫХ ЛЕКАРСТВЕННЫХ СРЕДСТВ

RESEARCH AND DEVELOPMENT OF NEW DRUG PRODUCTS

Comparative transcriptomic analysis of the malaria parasites Plasmodium falciparum and Plasmodium vivax sensitive and resistant strains

https://orcid.org/0000-0001-6939-1183

Sakpal

Shrutika Sakpal

Jaipur, 303002, Rajasthan, India

Mumbai, 400032, India

Shrutika Sakpal

Jaipur, 303002, Rajasthan, India

Mumbai, 400032, India

https://orcid.org/0000-0002-8077-1117

Abdeen

S. Z.

Abdeen

S. Z.

Sayyed Zara Abdeen

Mumbai, 400032, India

Sayyed Zara Abdeen

Mumbai, 400032, India

https://orcid.org/0000-0002-0378-4897

Kothari

Sh. L.

Kothari

Sh. L.

Shanker Lal Kothari

Jaipur, 303002, Rajasthan, India

Shanker Lal Kothari

Jaipur, 303002, Rajasthan, India

https://orcid.org/0000-0002-5036-8191

Bastikar

Virupaksha Bastikar

Maharashtra, 410206, India

Virupaksha Bastikar

Maharashtra, 410206, India

Amity Institute of Biotechnology, Amity University Rajasthan; Department of Biotechnology, Dr. Homi Bhabha State University, The Institute of ScienceAmity Institute of Biotechnology, Amity University Rajasthan; Department of Biotechnology, Dr. Homi Bhabha State University, The Institute of Science

Department of Biotechnology, Dr. Homi Bhabha State University, The Institute of ScienceDepartment of Biotechnology, Dr. Homi Bhabha State University, The Institute of Science

Amity Institute of Biotechnology, Amity University RajasthanAmity Institute of Biotechnology, Amity University Rajasthan

Amity Institute of Biotechnology, Amity UniversityAmity Institute of Biotechnology, Amity University

2022

22022022

1112331

2022

Sakpal S., Abdeen S.Z., Kothari S.L., Bastikar V.

Данная работа распространяется под лицензией Creative Commons Attribution 4.0.

This work is licensed under a Creative Commons Attribution 4.0 License.

https://www.pharmjournal.ru/jour/article/view/1155

Introduction

Introduction. Malaria is the sixth leading cause of death worldwide. According to a WHO survey (2019-2020), the total number of malaria deaths is estimated to be 409000. Plasmodium falciparum and Plasmodium vivax are major malaria parasites, particularly in subtropical areas.

Materials and methods

Materials and methods. In the present study, we used a transcriptome analysis of raw RNA sequence data to identify and characterize the differentially expressed genes in Plasmodium falciparum chloroquine-sensitive and chloroquine-resistant strains, as well as Plasmodium vivax primaquine sensitive and primaquine resistant strains. The raw RNA sequence data were obtained from the NCBI SRA database using the Accession IDs PRJNA308455, SRR14191963, and SRR332573.

Results and discussion

Results and discussion. The sequence of raw RNA was quantified, mapped, and annotated. The total number of reads mapped to the reference genome for Plasmodium falciparum was found to be 45474448 and for Plasmodium vivax was 38226870. The Cufflinks-Cuffdiff tool was used to identify differentially expressed genes in Plasmodium falciparum and Plasmodium vivax sensitive and resistant strains. This differentially expressed gene was further annotated and plotted using the "Limma" package of Bioconductor. The PPI network was constructed in String Database and Cytoscape software. Pathway enrichment analysis of list differentially expressed gene performed using KEGG and GO tool.

Conclusion

Conclusion. In sensitive and resistant strains, comparative transcriptome analysis revealed differentially regulated gene expression patterns.

Introduction

Materials and methods

Results and discussion

Conclusion

Conclusion. In sensitive and resistant strains, comparative transcriptome analysis revealed differentially regulated gene expression patterns.

Gene annotationKEGGMalariaProtein-protein interactionTranscriptome analysis

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The authors declare that there are no conflicts of interest present.