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<article article-type="research-article" dtd-version="1.3" xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xml:lang="ru"><front><journal-meta><journal-id journal-id-type="publisher-id">pharmjournal</journal-id><journal-title-group><journal-title xml:lang="ru">Разработка и регистрация лекарственных средств</journal-title><trans-title-group xml:lang="en"><trans-title>Drug development &amp; registration</trans-title></trans-title-group></journal-title-group><issn pub-type="ppub">2305-2066</issn><issn pub-type="epub">2658-5049</issn><publisher><publisher-name>LLC «CPHA»</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="doi">10.33380/2305-2066-2022-11-4(1)-31-37</article-id><article-id custom-type="elpub" pub-id-type="custom">pharmjournal-1392</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="ru"><subject>ПОИСК И РАЗРАБОТКА НОВЫХ ЛЕКАРСТВЕННЫХ СРЕДСТВ</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="en"><subject>RESEARCH AND DEVELOPMENT OF NEW DRUG PRODUCTS</subject></subj-group></article-categories><title-group><article-title>Фитостимулирующее действие продукта биодеструкции парацетамола на календулу лекарственную</article-title><trans-title-group xml:lang="en"><trans-title>Phyto-stimulating Effect of Paracetamol Biodestruction Product on Calendula Officinalis</trans-title></trans-title-group></title-group><contrib-group><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0002-7202-0073</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Вихарева</surname><given-names>Е. В.</given-names></name><name name-style="western" xml:lang="en"><surname>Vihareva</surname><given-names>E. V.</given-names></name></name-alternatives><bio xml:lang="ru"><p>614990, г. Пермь, ул. Полевая, д. 2</p></bio><bio xml:lang="en"><p>2, Polevaya str., Perm, 614990</p></bio><email xlink:type="simple">ajm@perm.ru</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0002-6496-7427</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Мишенина</surname><given-names>И. И.</given-names></name><name name-style="western" xml:lang="en"><surname>Mishenina</surname><given-names>I. I.</given-names></name></name-alternatives><bio xml:lang="ru"><p>614990, г. Пермь, ул. Полевая, д. 2</p></bio><bio xml:lang="en"><p>2, Polevaya str., Perm, 614990</p></bio><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0002-4626-3143</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Гапечкина</surname><given-names>Е. Д.</given-names></name><name name-style="western" xml:lang="en"><surname>Gapechkina</surname><given-names>E. D.</given-names></name></name-alternatives><bio xml:lang="ru"><p>614990, г. Пермь, ул. Полевая, д. 2</p></bio><bio xml:lang="en"><p>2, Polevaya str., Perm, 614990</p></bio><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0001-8773-6894</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Селянинов</surname><given-names>А. А.</given-names></name><name name-style="western" xml:lang="en"><surname>Selyaninov</surname><given-names>A. A.</given-names></name></name-alternatives><bio xml:lang="ru"><p>614990, г. Пермь, Комсомольский проспект, д. 29</p></bio><bio xml:lang="en"><p>29, Komsomolsky av., Perm, 614990</p></bio><xref ref-type="aff" rid="aff-2"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0002-6598-5870</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Рычкова</surname><given-names>М. И.</given-names></name><name name-style="western" xml:lang="en"><surname>Rychkova</surname><given-names>M. I.</given-names></name></name-alternatives><bio xml:lang="ru"><p>614990, г. Пермь, ул. Ленина, зд.13А</p></bio><bio xml:lang="en"><p>13A, Lenina str., Perm, 614990</p></bio><xref ref-type="aff" rid="aff-3"/></contrib></contrib-group><aff-alternatives id="aff-1"><aff xml:lang="ru"><institution>ФГБОУ ВО «Пермская государственная фармацевтическая академия» Министерства здравоохранения Российской Федерации (ФГБОУ ВО ПГФА Минздрава России)</institution></aff><aff xml:lang="en"><institution>Federal State Budgetary Educational Institution of Higher Education "Perm State Pharmaceutical Academy" of the Ministry of Health of the Russian Federation</institution></aff></aff-alternatives><aff-alternatives id="aff-2"><aff xml:lang="ru"><institution>ФГАОУ ВО «Пермский национальный исследовательский политехнический университет» (ПНИПУ)</institution></aff><aff xml:lang="en"><institution>Perm National Research Polytechnic University</institution></aff></aff-alternatives><aff-alternatives id="aff-3"><aff xml:lang="ru"><institution>ФГБУН «Пермский федеральный исследовательский центр Уральского отделения Российской академии наук» (ПФИЦ УрО РАН)</institution></aff><aff xml:lang="en"><institution>Perm Federal Research Center of the Ural Branch of the Russian Academy of Sciences</institution></aff></aff-alternatives><pub-date pub-type="collection"><year>2022</year></pub-date><pub-date pub-type="epub"><day>26</day><month>12</month><year>2022</year></pub-date><volume>11</volume><issue>4</issue><issue-title>Приложение 1</issue-title><fpage>31</fpage><lpage>37</lpage><permissions><copyright-statement>Copyright &amp;#x00A9; Вихарева Е.В., Мишенина И.И., Гапечкина Е.Д., Селянинов А.А., Рычкова М.И., 2022</copyright-statement><copyright-year>2022</copyright-year><copyright-holder xml:lang="ru">Вихарева Е.В., Мишенина И.И., Гапечкина Е.Д., Селянинов А.А., Рычкова М.И.</copyright-holder><copyright-holder xml:lang="en">Vihareva E.V., Mishenina I.I., Gapechkina E.D., Selyaninov A.A., Rychkova M.I.</copyright-holder><license xml:lang="ru" license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>Данная работа распространяется под лицензией Creative Commons Attribution 4.0.</license-p></license><license xml:lang="en" license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>This work is licensed under a Creative Commons Attribution 4.0 License.</license-p></license></permissions><self-uri xlink:href="https://www.pharmjournal.ru/jour/article/view/1392">https://www.pharmjournal.ru/jour/article/view/1392</self-uri><abstract><sec><title>Введение</title><p>Введение. В последние годы наблюдается нарастание фундаментального интереса к поиску эффективных, в том числе микробиологических, способов переработки фармацевтических отходов для получения на их основе новых биологически активных соединений. Полученные нами результаты показали, что продукт бактериальной деструкции парацетамола (ПБП) проявляет выраженные стимулирующие свойства в отношении лекарственных растений семейств Plantaginaceae, Lamiaceae, Urticaceae, Linaceae и может использоваться как индуктор накопления в них биологически активных веществ.</p></sec><sec><title>Цель</title><p>Цель. Цель настоящей работы – исследование влияния ПБП на динамику накопления биомассы и содержание флавоноидов в цветках календулы лекарственной (Calendula officinalis L.), сем. Астровые (Asteraceae) в сравнении с контролем (водой) и стимулятором роста «Циркон».</p></sec><sec><title>Материалы и методы</title><p>Материалы и методы. В работе использовали ПБП, полученный на базе лаборатории алканотрофных микроорганизмов ПФИЦ УрО РАН (Пермь). Цветки календулы лекарственной, собранные с растений, обработанных ПБП и стимулятором роста «Циркон», использовали для исследования динамики накопления сухой биомассы и изменения содержания флавоноидов спектрофотометрическим методом. Для сравнительного анализа интенсивности прироста биомассы цветков при обработке растений данными агентами, а также для прогноза накопления флавоноидов применили кинетическое моделирование.</p></sec><sec><title>Результаты и обсуждение</title><p>Результаты и обсуждение. Общий сбор биомассы цветков календулы лекарственной при обработке растений ПБП увеличился на 55 %, а при обработке стимулятором роста «Циркон» – на 24 % по сравнению с контролем. Содержание флавоноидов в цветках при обработке данными агентами увеличилось на 101 и 40 % соответственно. Определены сроки начала сбора цветков календулы лекарственной в условиях Западного Урала: при использовании стимулятора роста «Циркон» – с 20 июля, ПБП – с 1 августа, без обработки стимуляторами роста – с 14 августа. Оба стимулятора увеличивают дату окончания сбора сырья на 10 суток.</p></sec><sec><title>Заключение</title><p>Заключение. Продукт бактериальной деструкции парацетамола оказывает выраженное стимулирующее действие на календулу лекарственную (Calendula officinalis L.), существенно увеличивая биомассу цветков и содержание в них флавоноидов по сравнению с контролем (водой) и стимулятором роста «Циркон». С применением кинетического моделирования установлены значения параметров скорости роста биомассы растительного сырья при обработке растений данными агентами, осуществлен прогноз накопления флавоноидов и обоснованы сроки сбора цветков календулы лекарственной в условиях Западного Урала.</p></sec></abstract><trans-abstract xml:lang="en"><sec><title>Introduction</title><p>Introduction. In recent years, there has been an increase in fundamental interest in the search for effective, including microbiological, methods for processing pharmaceutical waste to obtain new biologically active compounds on their basis. Our results showed that the product of bacterial degradation of paracetamol (BDP) exhibits pronounced stimulating properties in relation to medicinal plants of the families Plantaginaceae, Lamiaceae, Urticaceae, Linaceae and can be used as an inducer of the accumulation of biologically active substances in them.</p></sec><sec><title>Aim</title><p>Aim. The purpose of this work is to study the effect of BDP on the dynamics of biomass accumulation and the content of flavonoids in the flowers of Calendula officinalis L., fam. Asteraceae in comparison with the control (water) and growth stimulator "Zircon".</p></sec><sec><title>Materials and methods</title><p>Materials and methods. BDP obtained on the basis of the Laboratory of Alkanotrophic Microorganisms Institute of Ecology and Genetics of Microorganism UB RAS (Perm). Calendula officinalis flowers collected from plants treated with BDP and Zircon growth stimulator were used to study the dynamics of dry biomass accumulation and changes in the content of flavonoids by the spectrophotometric method. For a comparative analysis of the intensity of the increase in the biomass of flowers during the treatment of plants with these agents, as well as to predict the accumulation of flavonoids, kinetic modeling was used.</p></sec><sec><title>Results and discussion</title><p>Results and discussion. The total biomass harvest of calendula officinalis flowers when treated with BDP increased by 55 %, and when treated with the Zircon growth stimulator, by 24 % compared to the control. The content of flavonoids in flowers when treated with these agents increased by 101 and 40 %, respectively. The dates for the beginning of the collection of calendula officinalis flowers in the conditions of the Western Urals were determined: with the use of the Zircon growth stimulator – from July 20, BDP – from August 1, without treatment with growth stimulants – from August 14. Both stimulants increase the end date of the collection of raw materials by 10 days.</p></sec><sec><title>Conclusion</title><p>Conclusion. The product of bacterial degradation of paracetamol has a pronounced stimulating effect on calendula officinalis, significantly increasing the biomass of flowers and the content of flavonoids in them compared to the control (water) and growth stimulator "Zircon". With the use of kinetic modeling, the values of the parameters of the growth rate of the biomass of plant raw materials during the treatment of plants with these agents were established, a forecast was made for the accumulation of flavonoids, and the timing of the collection of calendula officinalis flowers in the conditions of the Western Urals was substantiated.</p></sec></trans-abstract><kwd-group xml:lang="ru"><kwd>продукт биодеструкции парацетамола</kwd><kwd>фитостимуляция</kwd><kwd>календула лекарственная (Calendula officinalis L.)</kwd><kwd>флавоноиды</kwd><kwd>кинетическое моделирование</kwd></kwd-group><kwd-group xml:lang="en"><kwd>biodegradation product of paracetamol</kwd><kwd>phytostimulation</kwd><kwd>calendula officinalis</kwd><kwd>flavonoids</kwd><kwd>kinetic modeling</kwd></kwd-group><funding-group><funding-statement xml:lang="ru">Исследование проведено при финансовой поддержке Пермского научно-образовательного центра «Рациональное недропользование», 2022 год.</funding-statement><funding-statement xml:lang="en">The study was carried out with the financial support of the Perm Scientific and Educational Center "Rational Subsoil Use", 2022.</funding-statement></funding-group></article-meta></front><back><ref-list><title>References</title><ref id="cit1"><label>1</label><citation-alternatives><mixed-citation xml:lang="ru">Fekadu S., Alemayehu E., Dewil R., Van der Bruggen B. 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