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<article article-type="research-article" dtd-version="1.3" xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xml:lang="ru"><front><journal-meta><journal-id journal-id-type="publisher-id">pharmjournal</journal-id><journal-title-group><journal-title xml:lang="ru">Разработка и регистрация лекарственных средств</journal-title><trans-title-group xml:lang="en"><trans-title>Drug development &amp; registration</trans-title></trans-title-group></journal-title-group><issn pub-type="ppub">2305-2066</issn><issn pub-type="epub">2658-5049</issn><publisher><publisher-name>LLC «CPHA»</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="doi">10.33380/2305-2066-2023-12-3-111-117</article-id><article-id custom-type="elpub" pub-id-type="custom">pharmjournal-1549</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="ru"><subject>МЕТОДЫ АНАЛИЗА ЛЕКАРСТВЕННЫХ СРЕДСТВ</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="en"><subject>ANALYTICAL METHODS</subject></subj-group></article-categories><title-group><article-title>Сравнительное изучение анатомо-диагностических признаков листьев ежевики сизой (Rubus caesius L.), ежевики аллеганской (R. allegheniensis Porter) и ежевики несской (R. nessensis Hall)</article-title><trans-title-group xml:lang="en"><trans-title>Comparative Study of Anatomical and Diagnostic Features of the Leaves of the European Dewberry (Rubus caesius L.), Allegheny Blackberry (R. allegheniensis Porter) and European Blackberry (R. nessensis Hall)</trans-title></trans-title-group></title-group><contrib-group><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0003-1989-4545</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Ильина</surname><given-names>М. Б.</given-names></name><name name-style="western" xml:lang="en"><surname>Ilina</surname><given-names>M. B.</given-names></name></name-alternatives><bio xml:lang="ru"><p>119991, г. Москва, ул. Трубецкая, д. 8, стр. 2</p></bio><bio xml:lang="en"><p>8/2, Trubetskaya str., Mosсow, 119991</p></bio><email xlink:type="simple">rita221096@yandex.ru</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0002-7194-5525</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Сергунова</surname><given-names>Е. В.</given-names></name><name name-style="western" xml:lang="en"><surname>Sergunova</surname><given-names>E. V.</given-names></name></name-alternatives><bio xml:lang="ru"><p>119991, г. Москва, ул. Трубецкая, д. 8, стр. 2</p></bio><bio xml:lang="en"><p>8/2, Trubetskaya str., Mosсow, 119991</p></bio><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0003-1591-4019</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Бобкова</surname><given-names>Н. В.</given-names></name><name name-style="western" xml:lang="en"><surname>Bobkova</surname><given-names>N. V.</given-names></name></name-alternatives><bio xml:lang="ru"><p>119991, г. Москва, ул. Трубецкая, д. 8, стр. 2; 119192, г. Москва, Ломоносовский пр-т., д. 27, корп. 1</p></bio><bio xml:lang="en"><p>8/2, Trubetskaya str., Mosсow, 119991; 27/1, Lomonosovsky prospect, Moscow, 119192</p></bio><xref ref-type="aff" rid="aff-2"/></contrib></contrib-group><aff-alternatives id="aff-1"><aff xml:lang="ru"><institution>Федеральное государственное автономное образовательное учреждение высшего образования Первый Московский государственный медицинский университет имени И. М. Сеченова Министерства здравоохранения Российской Федерации (Сеченовский Университет)</institution></aff><aff xml:lang="en"><institution>I. M. Sechenov First MSMU of the Ministry of Health of the Russian Federation (Sechenov University)</institution></aff></aff-alternatives><aff-alternatives id="aff-2"><aff xml:lang="ru"><institution>Федеральное государственное автономное образовательное учреждение высшего образования Первый Московский государственный медицинский университет имени И. М. Сеченова Министерства здравоохранения Российской Федерации (Сеченовский Университет); Федеральное государственное бюджетное образовательное учреждение высшего образования «Московский государственный университет имени М. В. Ломоносова» (МГУ имени М. В. Ломоносова). Факультет фундаментальной медицины</institution></aff><aff xml:lang="en"><institution>I. M. Sechenov First MSMU of the Ministry of Health of the Russian Federation (Sechenov University); Lomonosov Moscow State University. Faculty of Fundamental Medicine</institution></aff></aff-alternatives><pub-date pub-type="collection"><year>2023</year></pub-date><pub-date pub-type="epub"><day>30</day><month>08</month><year>2023</year></pub-date><volume>12</volume><issue>3</issue><fpage>111</fpage><lpage>117</lpage><permissions><copyright-statement>Copyright &amp;#x00A9; Ильина М.Б., Сергунова Е.В., Бобкова Н.В., 2023</copyright-statement><copyright-year>2023</copyright-year><copyright-holder xml:lang="ru">Ильина М.Б., Сергунова Е.В., Бобкова Н.В.</copyright-holder><copyright-holder xml:lang="en">Ilina M.B., Sergunova E.V., Bobkova N.V.</copyright-holder><license xml:lang="ru" license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>Данная работа распространяется под лицензией Creative Commons Attribution 4.0.</license-p></license><license xml:lang="en" license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>This work is licensed under a Creative Commons Attribution 4.0 License.</license-p></license></permissions><self-uri xlink:href="https://www.pharmjournal.ru/jour/article/view/1549">https://www.pharmjournal.ru/jour/article/view/1549</self-uri><abstract><sec><title>Введение</title><p>Введение. Расширение номенклатуры фармацевтических субстанций, в том числе растительного происхождения, является одной из задач развития фармацевтической отрасли. Примером перспективного и доступного лекарственного растительного сырья могут служить листья ежевики сизой, обладающие доказанной противовоспалительной, гипогликемической, антимикробной активностью. Производящее растение – ежевика сизая (Rubus caesius) относится к роду Rubus, включающему множество видов, зачастую трудноразличимых между собой. Для полной фармакогностической характеристики лекарственного растительного сырья необходимо проведение микроскопического исследования.</p></sec><sec><title>Цель</title><p>Цель. Провести сравнительный микроскопический анализ листьев ежевики сизой (R. caesius L.), ежевики аллеганской (R. allegheniensis Porter) и ежевики несской (R. nessensis Hall) для установления анатомо-диагностических признаков.</p></sec><sec><title>Материалы и методы</title><p>Материалы и методы. Образцы листьев собраны на территории Москвы и Московской области в 2021–2022 гг. Анализ микроскопических структур проводился на световом микроскопе Leica DM1000 (Германия) с окуляром 10х/20 и объективами 10х/0,25 и 40х/0,65.</p></sec><sec><title>Результаты и обсуждение</title><p>Результаты и обсуждение. Проведен сравнительный анализ анатомо-диагностических структур листьев ежевики сизой и близких ей видов. Были исследованы размер и частота встречаемости на 1 мм2 простых и железистых волосков и устьиц, установлен тип устьичного аппарата, рассмотрены форма клеток эпидермы и кристаллические включения в мезофилле.</p></sec><sec><title>Заключение</title><p>Заключение. В результате проведенного исследования были выявлены различия в анатомических структурах листьев трех видов ежевики. Установлены анатомо-диагностические признаки листьев ежевики сизой, являющиеся показателями подлинности данного сырья, среди которых: наличие простых одноклеточных, сросшихся у основания волосков, обильное опушение листьев (особенно на нижней эпидерме), большое количество устьиц (106 ± 37 на 1 мм2), извилистая форма эпидермальных клеток, кубические кристаллы оксалата кальция в мезофилле.</p></sec></abstract><trans-abstract xml:lang="en"><sec><title>Introduction</title><p>Introduction. Expansion of the nomenclature of raw materials, including herbal substances, is one of the tasks of the development of the pharmaceutical industry. As an example of a potential and affordable medicinal plant raw material, we can offer the leaves of European dewberry with a proven anti-inflammatory, hypoglycemic, antimicrobial activity. Medicinal plant – European dewberry (Rubus caesius L.) belongs to the genus Rubus L., which includes many species, often difficult to distinguish between each other. For complete pharmacognostic characteristics of medicinal plant raw materials, microscopic examination is necessary.</p></sec><sec><title>Aim</title><p>Aim. To carry out a comparative microscopic analysis of the leaves of R. caesius L., R. allegheniensis Porter and R. nessensis Hall to establish anatomical and diagnostic characters.</p></sec><sec><title>Materials and methods</title><p>Materials and methods. Leaf samples were collected on the territory of Moscow and the Moscow region in 2021-2022. Microscopic structures were analyzed using a Leica DM1000 light microscope (Germany) with a 10x/20 eyepiece and 10x/0.25 and 40x/0.65 lenses.</p></sec><sec><title>Results and discussion</title><p>Results and discussion. A comparative analysis of the anatomical and diagnostic structures of the leaves of the Rubus caesius L. (European dewberry) and its related species has been carried out. The size and frequency of occurrence per 1 mm2 of simple and glandular hairs and stomata were investigated, the type of stomatal apparatus was established, the shape of epidermal cells and crystalline inclusions in the mesophyll were considered.</p></sec><sec><title> Conclusion</title><p> Conclusion. As a result of the study, differences in the anatomical structures of the leaves of three types of blackberries were revealed. Anatomical and diagnostic characters of Rubus caesius L. leaves have been established, which are indicators of the identification of this raw material, including: the presence of simple one-celled fused at the base trichomes, abundant leaf pubescence (especially on the abaxial epiderm), a large number of stomata (106 ± 37 per 1 mm2), the sinuous shape of epidermal cells, cubic crystals of calcium oxalate in the mesophyll.</p></sec></trans-abstract><kwd-group xml:lang="ru"><kwd>Rubus caesius L.</kwd><kwd>R. allegheniensis Porter</kwd><kwd>R. nessensis Hall</kwd><kwd>ежевика сизая</kwd><kwd>ежевика аллеганская</kwd><kwd>ежевика несская</kwd><kwd>листья ежевики</kwd><kwd>микроскопический анализ</kwd></kwd-group><kwd-group xml:lang="en"><kwd>Rubus caesius L.</kwd><kwd>R. allegheniensis Porter</kwd><kwd>R. nessensis Hall</kwd><kwd>European dewberry</kwd><kwd>Allegheny blackberry</kwd><kwd>European blackberry</kwd><kwd>blackberry leaves</kwd><kwd>microscopic analysis</kwd></kwd-group></article-meta></front><back><ref-list><title>References</title><ref id="cit1"><label>1</label><citation-alternatives><mixed-citation xml:lang="ru">Дунаева С. Е., Красовская Л. С., Гавриленко Т. А. Cохранение генетических ресурсов рода Rubus (Rosaceae) ex situ (обзор). Труды по прикладной ботанике, генетике и селекции. 2022;183(1):236–253.</mixed-citation><mixed-citation xml:lang="en">Dunaeva S. E., Krasovskaya L. S., Gavrilenko T. A. Conservation of genetic resources of the genus Rubus (Rosaceae) ex situ (review). Works on applied botany, genetics and breeding. 2022;183(1):236–253. (In Russ.)</mixed-citation></citation-alternatives></ref><ref id="cit2"><label>2</label><citation-alternatives><mixed-citation xml:lang="ru">Серегин А. П., ред. Цифровой гербарий МГУ. М.: МГУ; 2022.</mixed-citation><mixed-citation xml:lang="en">Seregin A. P., editor. Digital Herbarium of Moscow State University. Moscow: Moscow State University; 2022. (In Russ.)</mixed-citation></citation-alternatives></ref><ref id="cit3"><label>3</label><citation-alternatives><mixed-citation xml:lang="ru">Маевский П. Ф. Флора средней полосы европейской части России. 10-е изд. Москва: Товарищество научных изданий КМК; 2006. 600 с.</mixed-citation><mixed-citation xml:lang="en">Mayevsky P. F. Flora of the middle zone of the European part of Russia. 10th ed. Moscow: KMK Scientific Press; 2006. 600 p. (In Russ.)</mixed-citation></citation-alternatives></ref><ref id="cit4"><label>4</label><citation-alternatives><mixed-citation xml:lang="ru">Chaves V. C., Soares M. S., Spohr L., Teixeira F., Vieira A., Constantino L. S., Dal Pizzol F., Lencina C. L., Spanevello R. M., Freitas M. P., Simões C. M. O., Reginatto F. H., Stefanello F. M. Blackberry extract improves behavioral and neurochemical dysfunctions in a ketamine-induced rat model of mania. Neuroscience Letters. 2020;714:134566. DOI: 10.1016/j.neulet.2019.134566.</mixed-citation><mixed-citation xml:lang="en">Chaves V. C., Soares M. S., Spohr L., Teixeira F., Vieira A., Constantino L. S., Dal Pizzol F., Lencina C. L., Spanevello R. M., Freitas M. P., Simões C. M. O., Reginatto F. H., Stefanello F. M. Blackberry extract improves behavioral and neurochemical dysfunctions in a ketamine-induced rat model of mania. Neuroscience Letters. 2020;714:134566. DOI: 10.1016/j.neulet.2019.134566.</mixed-citation></citation-alternatives></ref><ref id="cit5"><label>5</label><citation-alternatives><mixed-citation xml:lang="ru">Paczkowska-Walendowska M., Gościniak A., Szymanowska D., Szwajgier D., Baranowska-Wójcik E., Szulc P., Dreczka D., Simon M., Cielecka-Piontek J. Blackberry Leaves as New Functional Food? Screening Antioxidant, Anti-Inflammatory and Microbiological Activities in Correlation with Phytochemical Analysis. Antioxidants. 2021;10(12):1945. DOI: 10.3390/antiox10121945.</mixed-citation><mixed-citation xml:lang="en">Paczkowska-Walendowska M., Gościniak A., Szymanowska D., Szwajgier D., Baranowska-Wójcik E., Szulc P., Dreczka D., Simon M., Cielecka-Piontek J. Blackberry Leaves as New Functional Food? Screening Antioxidant, Anti-Inflammatory and Microbiological Activities in Correlation with Phytochemical Analysis. Antioxidants. 2021;10(12):1945. DOI: 10.3390/antiox10121945.</mixed-citation></citation-alternatives></ref><ref id="cit6"><label>6</label><citation-alternatives><mixed-citation xml:lang="ru">Veličković I., Grujić S., Džamić A., Krivošej Z., Marin P. In vitro antioxidant activity of dewberry (Rubus caesius L. var. aquáticus Weihe. &amp; Nees.) leaf extracts. Archives of Biological Sciences. 2015;67(4):1323–1330. DOI: 10.2298/ABS150414109V.</mixed-citation><mixed-citation xml:lang="en">Veličković I., Grujić S., Džamić A., Krivošej Z., Marin P. In vitro antioxidant activity of dewberry (Rubus caesius L. var. aquáticus Weihe. &amp; Nees.) leaf extracts. Archives of Biological Sciences. 2015;67(4):1323–1330. DOI: 10.2298/ABS150414109V.</mixed-citation></citation-alternatives></ref><ref id="cit7"><label>7</label><citation-alternatives><mixed-citation xml:lang="ru">Hering A., Stefanowicz-Hajduk J., Hałasa R., Olech M., Nowak R., Kosiński P., Ochocka J. R. Polyphenolic Characterization, Antioxidant, Antihyaluronidase and Antimicrobial Activity of Young Leaves and Stem Extracts from Rubus caesius L. Molecules. 2022;27(19):6181. DOI: 10.3390/molecules27196181.</mixed-citation><mixed-citation xml:lang="en">Hering A., Stefanowicz-Hajduk J., Hałasa R., Olech M., Nowak R., Kosiński P., Ochocka J. R. Polyphenolic Characterization, Antioxidant, Antihyaluronidase and Antimicrobial Activity of Young Leaves and Stem Extracts from Rubus caesius L. Molecules. 2022;27(19):6181. DOI: 10.3390/molecules27196181.</mixed-citation></citation-alternatives></ref><ref id="cit8"><label>8</label><citation-alternatives><mixed-citation xml:lang="ru">Krzepiłko A., Prażak R., Święciło A. Chemical composition, antioxidant and antimicrobial activity of raspberry, blackberry and raspberry-blackberry hybrid leaf buds. Molecules. 2021;26(2):327. DOI: 10.3390/molecules26020327.</mixed-citation><mixed-citation xml:lang="en">Krzepiłko A., Prażak R., Święciło A. Chemical composition, antioxidant and antimicrobial activity of raspberry, blackberry and raspberry-blackberry hybrid leaf buds. Molecules. 2021;26(2):327. DOI: 10.3390/molecules26020327.</mixed-citation></citation-alternatives></ref><ref id="cit9"><label>9</label><citation-alternatives><mixed-citation xml:lang="ru">Robinson J. A., Bierwirth J. E., Greenspan P., Pegg R. B. Blackberry polyphenols: Composition, quantity, and health impacts from in vitro and in vivo studies. Journal of Food Bioactives. 2020;9. DOI: 10.31665/JFB.2020.9217.</mixed-citation><mixed-citation xml:lang="en">Robinson J. A., Bierwirth J. E., Greenspan P., Pegg R. B. Blackberry polyphenols: Composition, quantity, and health impacts from in vitro and in vivo studies. Journal of Food Bioactives. 2020;9. DOI: 10.31665/JFB.2020.9217.</mixed-citation></citation-alternatives></ref><ref id="cit10"><label>10</label><citation-alternatives><mixed-citation xml:lang="ru">Sultana N. Plants of genus Rubus as a source of pharmaceuticals. CPQ Nutrition. 2018;3(1):1–71.</mixed-citation><mixed-citation xml:lang="en">Sultana N. Plants of genus Rubus as a source of pharmaceuticals. CPQ Nutrition. 2018;3(1):1–71.</mixed-citation></citation-alternatives></ref><ref id="cit11"><label>11</label><citation-alternatives><mixed-citation xml:lang="ru">Schädler V., Dergatschewa S. Rubus caesius L. Leaves: Pharmacognostic analysis and the study of hypoglycemic activity. National Journal of Physiology, Pharmacy and Pharmacology. 2017;7(5):501. DOI: 10.5455/njppp.2017.7.1234224012017.</mixed-citation><mixed-citation xml:lang="en">Schädler V., Dergatschewa S. Rubus caesius L. Leaves: Pharmacognostic analysis and the study of hypoglycemic activity. National Journal of Physiology, Pharmacy and Pharmacology. 2017;7(5):501. DOI: 10.5455/njppp.2017.7.1234224012017.</mixed-citation></citation-alternatives></ref><ref id="cit12"><label>12</label><citation-alternatives><mixed-citation xml:lang="ru">Wu T., Gao Y., Guo X., Zhang M., Gong L. Blackberry and blueberry anthocyanin supplementation counteract high-fat-diet-induced obesity by alleviating oxidative stress and inflammation and accelerating energy expenditure. Oxidative medicine and cellular longevity. 2018;2018:1–9. DOI: 10.1155/2018/4051232.</mixed-citation><mixed-citation xml:lang="en">Wu T., Gao Y., Guo X., Zhang M., Gong L. Blackberry and blueberry anthocyanin supplementation counteract high-fat-diet-induced obesity by alleviating oxidative stress and inflammation and accelerating energy expenditure. Oxidative medicine and cellular longevity. 2018;2018:1–9. DOI: 10.1155/2018/4051232.</mixed-citation></citation-alternatives></ref><ref id="cit13"><label>13</label><citation-alternatives><mixed-citation xml:lang="ru">Муллажонова М. Т., Исматуллаева Ю. А., Имамалиев Б. А., Рахимова Г. К. Изучение противодиарейной активности плодов ежевики сизой, произрастающей на територии узбекистана. Farmatsevtika jurnali. 2017;4:83.</mixed-citation><mixed-citation xml:lang="en">Mullazhonova M. T., Ismatullayeva Yu. A., Imamaliev B. A., Rakhimova G. K. Study of antidiarrheal activity of blackberry fruits growing in the territory of uzbekistan. Farmatsevtika jurnali. 2017;4:83. (In Russ.)</mixed-citation></citation-alternatives></ref><ref id="cit14"><label>14</label><citation-alternatives><mixed-citation xml:lang="ru">Государственная фармакопея Российской Федерации. 14-е изд., Т. 2. Москва: Министерства здравоохранения Российской Федерации; 2018.</mixed-citation><mixed-citation xml:lang="en">State Pharmacopoeia of the Russian Federation. 14th ed. V. 2. Moscow: Ministry of Health of the Russian Federation; 2018. (In Russ.)</mixed-citation></citation-alternatives></ref><ref id="cit15"><label>15</label><citation-alternatives><mixed-citation xml:lang="ru">Попова М. В., Брежнева Т. А., Сливкин А. И., Шестакова Г. Ю. Сравнительное изучение морфолого-анатомических признаков листьев ежевики разных сортов. Современные тенденции развития технологий здоровьесбережения. 2019;277–284.</mixed-citation><mixed-citation xml:lang="en">Popova M. V., Brezhnev T. A., Slivkin A. I., Shestakova G. Yu. Comparative study of morphological and anatomical features of blackberry leaves of different varieties. Sovremennye tendencii razvitija tehnologij zdorov’esberezheniya. 2019;277–284. (In Russ.)</mixed-citation></citation-alternatives></ref><ref id="cit16"><label>16</label><citation-alternatives><mixed-citation xml:lang="ru">Koczka N., Stefanovits-Banyai E., Prokaj E. Element composition, total phenolics and antioxidant activity of wild and cultivated blackberry (Rubus fruticosus L.) fruits and leaves during the harvest time. Notulae Botanicae Horti Agrobotanici Cluj-Napoca. 2018;46(2):563–569. DOI: 10.15835/nbha46210993.</mixed-citation><mixed-citation xml:lang="en">Koczka N., Stefanovits-Banyai E., Prokaj E. Element composition, total phenolics and antioxidant activity of wild and cultivated blackberry (Rubus fruticosus L.) fruits and leaves during the harvest time. Notulae Botanicae Horti Agrobotanici Cluj-Napoca. 2018;46(2):563–569. DOI: 10.15835/nbha46210993.</mixed-citation></citation-alternatives></ref></ref-list><fn-group><fn fn-type="conflict"><p>The authors declare that there are no conflicts of interest present.</p></fn></fn-group></back></article>
