Введение

pharmjournal

Разработка и регистрация лекарственных средств

Drug development & registration

2305-20662658-5049

LLC «CPHA»

10.33380/2305-2066-2024-13-4-1728

pharmjournal-1959

Research Article

МЕТОДЫ АНАЛИЗА ЛЕКАРСТВЕННЫХ СРЕДСТВ

ANALYTICAL METHODS

Разработка методики жидкостной хроматографии для анализа масляного экстракта полыни цитварной

Development of a liquid chromatography method for the analysis of oil extract of artemisia cina

https://orcid.org/0000-0002-6944-5548

Ордабаева

С. К.

Ordabayeva

S. K.

160019, г. Шымкент, пл. Аль-Фараби, д. 1

1, Al-Farabi square, Shymkent, 160019

https://orcid.org/0000-0002-4803-8708

Серикбаева

А. Д.

Serikbayeva

A. D.

160019, г. Шымкент, пл. Аль-Фараби, д. 1

1, Al-Farabi square, Shymkent, 160019

https://orcid.org/0000-0003-1356-385X

Фаршад

Ш. Х.

Farshad

H. S.

Тегеран, Веленджак, бульвар Данешджу, проспект Араби, корпус № 2 СБМУ

Bldg No. 2 SBMU, Arabi Ave, Daneshjoo Blvd, Velenjak, Tehran

https://orcid.org/0000-0002-9012-6584

Бахыткызы

Г.

Bakhytkyzy

160019, г. Шымкент, пл. Аль-Фараби, д. 1

1, Al-Farabi square, Shymkent, 160019

bakhytkyzy00@bk.ru

Акционерное общество «Южно-Казахстанская медицинская академия» (АО «ЮКМА»)JSC "South Kazakhstan Medical Academy"

Медицинский университет Шахида Бехешти, фармацевтический факультет, кафедра фармацевтической и токсикологической химииShahid Beheshti Medical University, School of Pharmacy, Department of pharmaceutical and toxicological chemistry

2024

01112024

134139147

2024

Ордабаева С.К., Серикбаева А.Д., Фаршад Ш.Х., Бахыткызы Г.

Ordabayeva S.K., Serikbayeva A.D., Farshad H.S., Bakhytkyzy G.

Данная работа распространяется под лицензией Creative Commons Attribution 4.0.

This work is licensed under a Creative Commons Attribution 4.0 License.

https://www.pharmjournal.ru/jour/article/view/1959

Введение

Введение. Проблема разработки и внедрения отечественных препаратов в Казахстане на сегодняшний день является острой и актуальной. Решение данной проблемы возможно посредством использования собственных ресурсов – отечественного лекарственного растительного сырья. Перспективным лекарственным сырьем является полынь цитварная – эндемичное лекарственное растение Туркестанской области Казахстана.

Цель

Цель. Разработка методики жидкостной хроматографии для анализа масляного экстракта полыни цитварной.

Материалы и методы

Материалы и методы. Стандартный образец (СО) сантонина масляный экстракт полыни цитварной (МЭПЦ). Идентификацию и количественное определение вещества проводили на жидкостном хроматографе Agilent Technologies 1200 (США) с программным обеспечением ChemStation.

Результаты и обсуждение

Результаты и обсуждение. Исследование хроматографического поведения основного действующего вещества масляного экстракта проводилось методом высокоэффективной жидкостной хроматографии (ВЭЖХ). Подбор условий разделения на колонке включает в себя выбор оптимального состава подвижной фазы и скорости элюирования. Использовалась обращенно-фазовая система с сорбентом C18 и подвижной фазой, состоящей из ацетонитрила и фосфатного буфера с рН 6,8. Хроматографические исследования субстанции проводили в градиентном режиме при аналитической длине волны 237 нм. Время удерживания стандартного образца (СО) сантонина совпало со временем удерживания (tR) сантонина выделенного из масляного экстракта полыни цитварной и составило 14,3 мин.

Заключение

Заключение. Разработана методика идентификации и количественного определения масляного экстракта по основному действующему веществу – сантонина методом жидкостной хроматографии.

Introduction

Introduction. The problem of development and implementation of own drugs in Kazakhstan today is acute and urgent. The solution to this problem is possible through the use of own resources – domestic medicinal plant raw materials. A promising medicinal raw material is Artemisia cina – an endemic medicinal plant of the of the Turkestan region of Kazakhstan.

Aim

Aim. Designing a liquid chromatography methodology to analyze the oil extract obtained from Artemisia cina.

Materials and methods

Materials and methods. Standard sample (SS) of santonin, Artemisia cina’s oil extract. The substance was identified and quantitatively determined using an Agilent Technologies 1200 liquid chromatograph (USA) equipped with ChemStation software.

Results and discussion

Results and discussion. The main active substance of the oil extract was analyzed using high-performance liquid chromatography (HPLC) method. Choosing the separation conditions for the column involves determining the best composition of the mobile phase and the rate of elution. There were used a reversed-phase system with sorbent C18 and a mobile phase consisting of acetonitrile and a phosphate buffer with a pH of 6.8. Chromatographic studies of the substance were carried out in a gradient mode at an analytical wavelength of 237 nm. The retention time of the standard sample (SS) of santonin matched the retention time (tR) of santonin isolated from the oil extract of Artemisia cina and amounted to 14.3 minutes.

Conclusion

Conclusion. A liquid chromatography method has been established for the identification and quantitative determination of the oil extract, focusing on the main active substance, santonin.

масляный экстракт полыни цитварнойсантонинидентификацияанализвысокоэффективная жидкостная хроматографиявалидация

oil extract of artemisia cinasantoninidentificationassayhigh-performance liquid chromatographyvalidation

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The authors declare that there are no conflicts of interest present.