pharmjournalРазработка и регистрация лекарственных средствDrug development & registration2305-20662658-5049LLC «CPHA»pharmjournal-550Research ArticleАНАЛИТИЧЕСКИЕ МЕТОДИКИ И МЕТОДЫ КОНТРОЛЯANALYTICAL AND QUALITY CONTROL METHODSСОВРЕМЕННЫЕ МЕТОДЫ АНАЛИЗА ОКИСЛЕННОГО И ВОССТАНОВЛЕННОГО КОЭНЗИМА Q₁₀ В БИОМАТЕРИАЛЕ (ОБЗОР)THE MODERN METHODS OF ANALYSIS OF OXIDIZED AND REDUCED FORMS OF COENZYME Q₁₀ IN BIOMATERIAL (REVIEW)ЗозинаВ. И.ZozinaV. I.noemail@neicon.ruГорошкоО. А.GoroshkoO. A.noemail@neicon.ruКрасныхЛ. М.KrasnykhL. M.noemail@neicon.ruКукесВ. Г.KukesV. G.noemail@neicon.ruФГАОУ ВО «Первый МГМУ им И.М. Сеченова» Минздрава России (Сеченовский Университет)I.M. Sechenov First Moscow State Medical University of the Ministry of Health of the Russian FederationФГБУ «Научный центр экспертизы средств медицинского применения» Министерства здравоохранения Российской ФедерацииScientific Centre for Expert Evaluation of Medical Products of the Ministry of Health of the Russian FederationФГАОУ ВО «Первый МГМУ им И.М. Сеченова» Минздрава России (Сеченовский Университет)I.M. Sechenov First Moscow State Medical University of the Ministry of Health of the Russian Federation; Scientific Centre for Expert Evaluation of Medical Products of the Ministry of Health of the Russian Federation201809012019018488Copyright © Зозина В.И., Горошко О.А., Красных Л.М., Кукес В.Г., 20192019Зозина В.И., Горошко О.А., Красных Л.М., Кукес В.Г.Zozina V.I., Goroshko O.A., Krasnykh L.M., Kukes V.G.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.pharmjournal.ru/jour/article/view/550

В организме человека коэнзим Q₁₀ находится в окисленной и восстановленной формах и обнаруживается во всех органах. В обзоре рассмотрены современные методики ВЭЖХ-анализа коэнзима Q₁₀ с использованием электрохимического, спектрофотометрического и масс-спектрометрического детекторов. В статье представлены данные по отбору проб, подготовке биоматериала к анализу и обеспечению стабильности коэнзима Q₁₀ в биоматериале. Проведен сравнительный анализ используемых методик определения для окисленной и восстановленной формы коэнзима Q₁₀ по чувствительности и селективности методов.

In the human body, coenzyme Q10 is in the oxidized and reduced forms and can be found in every organ. The review describes modern HPLC methods of coenzyme Q10 analysis using electrochemical, spectrophotometric and mass spectrometric detectors. In the article we present information on sampling, preparation of biomaterial for analysis and preservation of coenzyme Q10 stability in biomaterial. It is carried out a comparative analysis of the determination methodologies for the oxidized and reduced forms of coenzyme Q10 by its sensitivity and selectivity.

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The authors declare that there are no conflicts of interest present.