Comparison of the effects of copper chlorophyllin, riboxin, indralin and the combined use of glutathione and ascorbic acid on the survival of mice during fractionated X-ray irradiation

Introduction. The problem of effectiveness and safe pharmacological means of reducing the consequences of exposure to the ionizing effect is becoming increasingly urgent. This solution is difficult due to the high chemical toxicity of all known modern effective radioprotectors. Nowadays, much attention is paid to the study of the radioprotective properties of the so-called effects. But in most of these works, the experimental model was subjected to a one-time acute irradiation. At the same time, a safe and effective radioprotective drug under conditions of fractionated irradiation will be useful in radiation therapy for oncological diseases and during space missions, as well as in conditions of radiation contamination of territories.

Aim. Comparison of the radioprotective effect of copper chlorophyllin, riboxin, also called inosine, and the combined use of glutathione and ascorbic acid with that of the reference Russian radioprotector indralin during fractionated exposure to X-ray radiation.

Materials and methods. Male ICR (CD-1) mice were exposed to five daily irradiations of 2.5 Gy. On the days of each irradiation, experimental animals were administered chlorophyllin (20 μg/g), indralin (50 μg/g) in a solution of tartaric acid or glutathione (250 μg/g) and ascorbic acid (150 μg/g) before irradiation or riboxin (200 μg/g) after irradiation. The survival of mice was assessed within 30 days after the last irradiation.

Result and discussion. Only the use of Riboxin ensured the survival of 10 % of irradiated animals, but without a statistically significant increase in the average life expectancy of dead animals relative to the group of intact mice. A significant increase in this parameter was provided only by the use of indralin. Copper chlorophyllin had no radioprotective effect. Perhaps the use of metal-free chlorophyll derivatives in the future will be able to have a radioprotective effect under these conditions. The combined use of glutathione and ascorbic acid led to the death of 2 out of 10 mice during the period of irradiation and drug administration, without providing an increase in survival during the observation period.

Conclusion. The use of copper chlorophyllin and glutathione with ascorbic acid did not increase the survival rate and average life expectancy of deceased mice irradiated at a dose of 12.5 Gy, distributed into 5 fractions of 2.5 Gy. The use of indralin only increased the life expectancy of dead animals. Riboxin contributed to the survival of 10 % of the corresponding group.

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