Validation of the HPLC-UV Method for Quantitative Determination of Steroid Sapogenin Diosgenin from Hay Fenugreek Seeds, Trigonella Foenum-graecum L.
https://doi.org/10.33380/2305-2066-2020-9-4-107-114
Abstract
Introduction. Modern pharmacognostic research is aimed at searching for plant biologically active individual compounds (hereinafter referred to as RBAIS) isolated from plant extracts.
Aim. Validation of HPLC-UV quantitative determination of sapogenin diosgenin in plant extracts from fenugreek seeds.
Materials and methods. The object of study was raw materials-fenugreek seeds produced as medicinal plant raw materials by LLC «Sage» (Irkutsk). Validation of the method was carried out according to the parameters: specificity, linearity, correctness, precision in accordance with the requirements of SP XIV. One series of medicinal plant raw materials was used for the analysis, such as serial number – 010117, release date – 15 february 2017.
Results and discussion. Validation characteristics were determined and their compliance with the necessary acceptance criteria was experimentally confirmed.
Conclusion. It is established that the developed method of identification and quantitative determination of diosgenin in fenugreek seed extracts by HPLC-UV is correct, precise, specific and linear in the analytical field.
About the Authors
A. E. Sukhanov
Northern State Medical University
Russian Federation
Russian Federation
Anton E. Sukhanov
Faculty of Pharmacy, Department of Pharmacy and Pharmacology
51, Troitsky av., Arkhangelsk, 163000
A. N. Stavrianidi
Lomonosov Moscow State University
Russian Federation
Russian Federation
Andrey N. Stavrianidi
Faculty of Chemistry, Department of Analytical Chemistry
1/3, Leninskie Gory, GSP-1, Moscow, 119991
E. D. Kubasova
Northern State Medical University
Russian Federation
Russian Federation
Elena D. Kubasova
Faculty of Pharmacy, Department of Pharmacy and Pharmacology
51, Troitsky av., Arkhangelsk, 163000
A. S. Panasyuk
Northern State Medical University
Russian Federation
Russian Federation
Alexandra S. Panasyuk
Faculty of Pharmacy, Department of Pharmacy and Pharmacology
51, Troitsky av., Arkhangelsk, 163000
O. V. Buyuklinskaya
Northern State Medical University
Russian Federation
Russian Federation
Olga V. Buyuklinskaya
Faculty of Pharmacy, Department of Pharmacy and Pharmacology
51, Troitsky av., Arkhangelsk, 163000
References
1. Stekolshchikova E. A., Stavrianidi A. N, Porotova A., Rodin I., Shpigun O. Combination of HPLC–MS and QAMS as a new analytical approach for determination of saponins in ginseng containing products. Journal of Pharmaceutical and Biomedical Analysis. 2017;132:87–92. DOI: 10.1016/j.jpba.2016.09.041.
2. Stavrianidi A. N., Stekolshchikova, E. A., Turova, P. N., Rodin I. A., Shpigun O. A. Quantitative analysis of a multicomponent system for liquid chromatography–mass spectrometry determination of diosgenin, dioscin and protodioscin in plant extracts of Tribulus terrestris, 2017. Moscow University Chemistry Bulletin. 2017;72:144– 153. DOI: 10.3103/S0027131417030063.
3. Choi S. J. et al. Application of high-performance countercurrent chromatography for the isolation of steroidal saponins from Liriope plathyphylla. Journal of separation science. 2015;38(1):18–24. DOI: 10.1002/jssc.201401007.
4. Sarvin B., Stekolshikova E., Rodin I., Stavrianidi A., Shpigun O. Optimization and comparison of different techniques for complete extraction of saponins from T. terrestris. Journal of applied research on medicinal and aromatic plants. 2018;8:75–82. DOI: 10.1016/j.jarmap.2017.12.002.
5. Epstein N. A. Validation of analytical methods: graphical and computational criteria for evaluating the linearity of methods in practice. Razrabotka i registratsiya lekarstvennykh sredstv = Drug development & registration. 2019;8(2):122–130. (In Russ.). DOI: 10.33380/2305-2066-2019-8-2-122-130.
Review
For citations:
Sukhanov A.E., Stavrianidi A.N., Kubasova E.D., Panasyuk A.S., Buyuklinskaya O.V. Validation of the HPLC-UV Method for Quantitative Determination of Steroid Sapogenin Diosgenin from Hay Fenugreek Seeds, Trigonella Foenum-graecum L. Drug development & registration. 2020;9(4):107-114. (In Russ.) https://doi.org/10.33380/2305-2066-2020-9-4-107-114
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