Development of Technology of Phytosubstantiation of Silybum marianum L. Fruits as a Component of Complex Therapy of Liver Diseases
https://doi.org/10.33380/2305-2066-2023-12-4-1582
Abstract
Introduction. Currently, the problem of liver diseases is widespread among the population, especially non-alcoholic fatty liver disease, as well as medicinal liver lesions. In this regard, there is a need to find new solutions in the development of hepatotropic therapy drugs. In clinical practice, several hepatoprotective agents are often used simultaneously in the form of separate drugs or combinations. The combined use of substances in one finished dosage form (FDF) can provide both an enhancement of a particular pharmacological effect and an expansion of the spectrum of hepatotropic action. Components of plant origin are often introduced into the composition of combined hepatoprotectors. Silybum marianum L. fruits contain at least 2.4 % of the amount of flavolignans in terms of silybin, which causes the hepatoprotective effect of medicines based on them and explains the prospects of using this vegetable raw material in the treatment of liver diseases.
Aim. Development of technology for obtaining a dry extract of Silybum marianum L. fruit, enriched with silybin.
Materials and methods. The objects of the study were Silybum marianum (L.) Gaertn. fruits from producer 2, harvested in June 2022. The quality indicators of the finished product, a dry extract of Silybum marianum (L.) fruit, were determined by the methods described in the SP XIV ed. Statistical processing of the research results was carried out in accordance with SP XIV ed., Volume I, p. 289, GM.1.1.0013.15 "Statistical processing of the results of a chemical experiment".
Results and discussion. The optimal method of extraction of medicinal plant raw materials is proposed, which allows to obtain the highest yield of biologically active substances. A method of purification of ethanol-water extraction after extraction is proposed. The technology of dry extract of Silybum marianum (L.) fruit has been developed. Standardization of the obtained dry extract was carried out.
Conclusion. In the course of the study, the technology of dry extract of Silybum marianum (L.) fruit was developed. The quality indicators of phytosubstantiation, dry extract of Silybum marianum (L.) fruits enriched with silybin were determined.
About the Authors
B. A. PeresRussian Federation
14A, Prof. Popova str., Saint-Petersburg, 197022
A. P. Procyuk
Russian Federation
14A, Prof. Popova str., Saint-Petersburg, 197022
A. B. Zelencova
Russian Federation
14A, Prof. Popova str., Saint-Petersburg, 197022
I. E. Kauhova
Russian Federation
14A, Prof. Popova str., Saint-Petersburg, 197022
M. V. Aroyan
Russian Federation
14A, Prof. Popova str., Saint-Petersburg, 197022
E. K. Novikova
Russian Federation
14A, Prof. Popova str., Saint-Petersburg, 197022
References
1. Wang X., Zhang Z., Wu S. C. Health Benefits of Silybum marianum: Phytochemistry, Pharmacology, and Applications. Journal of Agricultural and Food Chemistry. 2021;68(42):11644–11664. DOI: 10.1021/acs.jafc.0c04791.
2. Kovanskov V. V., Ivkin D. Yu., Semivelichenko E. D., Plisko G. A., Kopacheva E. A., Sidorov K. O. Approbation of a model of fat liver disease induced by orotic acid. Drug development & registration. 2022;11(4):240–245. (In Russ.) DOI: 10.33380/2305-2066-2022-11-4-240-245.
3. Bunjat A. V., Spasenkova O. M., Karev V. E., Karavaeva A. V., Ivkin D. Yu., Kulikov A. N., Okovityi S. V., Kirillova N. V. Modification of a model of non-alcoholic fat liver disease in rats with a сombination of a hypercaloric diet and hypodynamia. Drug development & registration. 2021;10(4–1):155–165. (In Russ.) DOI: 10.33380/2305-2066-2021-10-4(1)-155-165.
4. Okovityj S. V., Rajhel’son K. L., Prihod’ko V. A. Combined hepatoprotective pharmacotherapy of liver diseases. Experimental & clinical gastroenterology. 2022;203(7):5–20. DOI: 10.31146/1682-8658-ecg-203-7-5-20. (In Russ.)
5. Kaukhova I. E., Basevich A. V., Abrosimova O. N., Vainshtain V. A. Herbal medicines. Technology, compositions, standardization: monography. Saint-Petersburg: Knorus publishing; 2019. 396 р. (In Russ.)
6. Křen V., Valentová K. Silybin and its congeners: from traditional medicine to molecular effects. Natural Product Reports. 2022;39(6):1264–1281. DOI: 10.1039/d2np00013j.
7. Gillessen A. Silymarin as Supportive Treatment in Liver Diseases: A Narrative Review. Annals of Internal Medicine. 2020;37(1):1279–1301. DOI: 10.1007/s12325-020-01251-y.
8. Aghemo A., Alekseeva O. P., Angelico F., Bakulin I. G., Bakulina N. V., Bordin D., Bueverov A. O., Drapkina O. M., Gillessen A., Kagarmanova E. M., Korochanskaya N. V., Kucheryavii U. A., Lazebnik L. B., Livzan M. A., Maev I. V., Martynov A. I., Osipenko M. F., Sas E. I., Starodubova A., Uspensky Y. P., Vinnitskaya E. V., Yakovenko E. P., Yakovlev A. A. Role of silymarin as antioxidant in clinical management of chronic liver diseases: a narrative review. Annals of Internal Medicine. 2022;54(1):1548–1560. DOI:10.1080/07853890.2022.2069854.
9. Zhang C. Y., Liu S., Yang M. Antioxidant and anti-inflammatory agents in chronic liver diseases: Molecular mechanisms and therapy. World Journal of Hepatology. 2023;15(2):180–200. DOI: 10.4254/wjh.v15.i2.180.
10. Gohari Mahmoudabad A., Gheybi F., Mehrabi M., Masoudi A., Mobasher Z., Vahedi H., Gharravi A. M., Bitaraf F. S., Rezayat Sorkhabadi S. M. Synthesis, characterization and hepatoprotective effect of silymarin phytosome nanoparticles on ethanol-induced hepatotoxicity in rats. Bioimpacts. 2023;13(4):301-311. DOI: 10.34172/bi.2023.24128.
11. Torre A. Silymarin in the management of liver enzyme activity in steatohepatitis: a case report. Drugs in Context. 2023;12:1–5. DOI: 10.7573/dic.2023-1-5.
12. Islam A., Mishra A., Siddiqui M. A., Siddiquie S. Recapitulation of Evidence of Phytochemical, Pharmacokinetic and Biomedical Application of Silybin. Drug research (Stuttgart). 2021;71(9):489–503. DOI: 10.1055/a-1528-2721.
13. Kurkin V. A., Lebedev A. A., Avdeeva E. V., Zapesochnaya G. G., Pervushkin S. V., Simerzina L. V., Bulatova M. V. A method for obtaining Silybum marianum L. extract. Patent RUS № 2102999. 27.01.1998. Available at: https://patents.google.com/patent/RU2102999C1/ru. Accessed: 03.06.2023. (In Russ.)
14. State Pharmacopoeia XIV edition. Moscow: Ministry of Health of the Russian Federation; 2018. (In Russ.)
15. Saleh I. A., Vinatoru М., Mason T. J., Abdel-Azim N. S., Aboutab E. A., Hammouda F.M. Ultrasonic-Assisted Extraction and Conventional Extraction of Silymarin from Silybum marianum seeds. Research Journal of Pharmaceutical. 2015;6(2):709–717.
Supplementary files
|
1. Графический абстракт | |
Subject | ||
Type | Other | |
View
(2MB)
|
Indexing metadata ▾ |
Review
For citations:
Peres B.A., Procyuk A.P., Zelencova A.B., Kauhova I.E., Aroyan M.V., Novikova E.K. Development of Technology of Phytosubstantiation of Silybum marianum L. Fruits as a Component of Complex Therapy of Liver Diseases. Drug development & registration. 2023;12(4):101-110. (In Russ.) https://doi.org/10.33380/2305-2066-2023-12-4-1582