Разработка методики жидкостной хроматографии для анализа масляного экстракта полыни цитварной

Введение. Проблема разработки и внедрения отечественных препаратов в Казахстане на сегодняшний день является острой и актуальной. Решение данной проблемы возможно посредством использования собственных ресурсов – отечественного лекарственного растительного сырья. Перспективным лекарственным сырьем является полынь цитварная – эндемичное лекарственное растение Туркестанской области Казахстана.

Цель. Разработка методики жидкостной хроматографии для анализа масляного экстракта полыни цитварной.

Материалы и методы. Стандартный образец (СО) сантонина масляный экстракт полыни цитварной (МЭПЦ). Идентификацию и количественное определение вещества проводили на жидкостном хроматографе Agilent Technologies 1200 (США) с программным обеспечением ChemStation.

Результаты и обсуждение. Исследование хроматографического поведения основного действующего вещества масляного экстракта проводилось методом высокоэффективной жидкостной хроматографии (ВЭЖХ). Подбор условий разделения на колонке включает в себя выбор оптимального состава подвижной фазы и скорости элюирования. Использовалась обращенно-фазовая система с сорбентом C18 и подвижной фазой, состоящей из ацетонитрила и фосфатного буфера с рН 6,8. Хроматографические исследования субстанции проводили в градиентном режиме при аналитической длине волны 237 нм. Время удерживания стандартного образца (СО) сантонина совпало со временем удерживания (t_R) сантонина выделенного из масляного экстракта полыни цитварной и составило 14,3 мин.

Заключение. Разработана методика идентификации и количественного определения масляного экстракта по основному действующему веществу – сантонина методом жидкостной хроматографии.

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