Development and evaluation of the quality of phytosomes with ecdysteroids Seratula coronata L.
https://doi.org/10.33380/2305-2066-2025-14-3-2072
Abstract
Introduction. Phytosomes containing a complex of plant components with phospholipids are highly soluble in lipid and aqueous media, overcome skin barriers well, and enhance the bioavailability of substances with low solubility. For phytoecdysteroids, substances with a wide range of pharmacological actions in the course of biopharmaceutical research, the prospects of creating nanoscale liposomal forms have been shown.
Aim. Development of conditions for obtaining phytosomes with ecdysteroids of Serratulae coronatae L. and assessment of their quality.
Materials and methods. The amount of ecdysteroids isolated from Serratulae coronatae L., the main of which is 20-hydroxyecdysone (at least 75 %). Large multilayer vesicles were obtained by forming a lipid film with further homogenization in an ultrasonic bath. The particle size, polydispersity index, zeta potential (ζ-potential) were determined on the device Zetasizer Nano ZS (Malvern Instruments Ltd., United Kingdom). The degree of inclusion of the active component was estimated by HPLC.
Results and discussion. The composition has been developed and technological stages have been proposed to obtain phytosomes (small single-layer vesicles) with a diameter of 130–170 nm and an IP ≤ 0.3. The formation of hydrogen bonds in the structure of phytosomes with the main ecdysteroid of Cimicifuga racemosa, 20-hydroxyecdysone, has been confirmed using NMR spectrometry. The degree of encapsulation of Echinops ritro ecdysteroids in phytosomes was 88 and 84 % after one year of storage.
Conclusion. The technology of phytosomes with ecdysteroids of the Serratulae coronatae L. has been developed. The stability of the resulting phytosomal complex indicates the possibility of creating soft dosage forms based on it.
About the Authors
E. I. Molokhova
Federal State Budgetary Educational Institution of Higher Education "Perm State Pharmaceutical Academy" of the Ministry of Health of the Russian Federation
Russian Federation
Russian Federation
2, Polevaya str., Perm, Perm region, 614990
D. E. Lipin
Federal State Budgetary Educational Institution of Higher Education "Perm State Pharmaceutical Academy" of the Ministry of Health of the Russian Federation; LLC "Izvarino Pharma"
Russian Federation
Russian Federation
2, Polevaya str., Perm, Perm region, 614990
1/1, 5th km, Vnukovskoye shosse, Vnukovskoye settlement, Moscow, 108817
Yu. V. Sorokina
Federal State Budgetary Educational Institution of Higher Education "Perm State Pharmaceutical Academy" of the Ministry of Health of the Russian Federation
Russian Federation
Russian Federation
2, Polevaya str., Perm, Perm region, 614990
V. G. Luzhanin
Federal State Budgetary Educational Institution of Higher Education "Perm State Pharmaceutical Academy" of the Ministry of Health of the Russian Federation
Russian Federation
Russian Federation
2, Polevaya str., Perm, Perm region, 614990
References
1. Solovieva A. G., Erimbetov K. T., Obvintseva O. V., Fedorova A. V., Mikhailov V. V. Physiological mechanisms of action and the prospects for the use of phytoecdysteroids in biomedical technologies. Problems of Productive Animal Biology. 2021;1:26–40. (In Russ.) DOI: 10.25687/1996-6733.prodanimbiol.2021.1.26-40.
2. Molohova E. I., Lipin D. E., Sorokina Yu. V. The main directions of pharmaceutical development of phytoecdysteroid preparations (review). Problems of biological, medical and pharmaceutical chemistry. 2022;25(8):3−9. (In Russ.) DOI: 10.29296/25877313-2022-08-01.
3. Erimbetov K. T., Fedorova A. V., Goncharova A. Ya., Bondarenko E. V. Creation of a nanoscale form of 20-hydroxyecdysone and research of its biological availability. Problems of Productive Animal Biology. 2020;3:106–113. (In Russ.) DOI: 10.25687/1996-6733.prodanimbiol.2020.3.106-113.
4. Novikova A. A., Kezimana P., Stanishevskiy Ya. M. Methods for obtaining liposomes, used as drug delivery systems (review). Drug development & registration. 2017;(2):134–138. (In Russ.)
5. Gorbik V. S., Shprakh Z. S., Kozlova Z. M., Salova V. G. Liposomes as a targeted delivery system of drugs (review). Russian Journal of Biotherapy. 2021;20(1):33–41. (In Russ.) DOI: 10.17650/1726-9784-2021-20-1-33-41.
6. Aini P. N., Muhammad D., Eko P. G., Rachmat M., Fahmi E. Formulation, Characterization and Antioxidant Myricetin Nanophytosome for Topical Delivery. Asian Journal of Pharmaceutical Research and Development. 2020;8(3):09–13. DOI: 10.22270/ajprd.v8i3.718.
7. Zverev Ya. F., Rykunova A. Ya. Modern nanocarriers as a factor in increased bioavailability and pharmacological activity of flavonoids. Biotechnology. 2022;38(1):47–68. (In Russ.) DOI: 10.56304/S0234275822010100.
8. Petrishina T. V., Chernova I. D., Voskanjan O. S. The use of rosemary essential oil liposomes to transport biologically active molecules into cosmetic hair products. Trends in the development of science and education. 2019;55(4):37–40. (In Russ.) DOI: 10.18411/lj-10-2019-63.
9. Sazhina N. N., Lapshin P. V., Zagoskina N. V., Palmina N. P. Inhibition of phosphatidylcholine liposomes oxidation by phenolic compounds of aloe extracts: A. arborescens, A. pillansii and A. squarrosa. Chemistry of plant raw materials. 2019;2:83–90. (In Russ.) DOI: 10.14258/jcprm.2019024885.
10. Poltavec Yu. I., Kuznecov S. L., Tubasheva I. A., Muraveva A. I., Gukasova N. V. Nano- and micro-sized forms of silymarin and silybin. Russian nanotechnology. 2021;16(2):149– 174. (In Russ.) DOI: 10.1134/S1992722321020102.
11. Feldman N. B., Orekhov S. N., Chakaleva I. I., Muchkinova E. A., Sedyakina N. E., Lutsenko S. V. Preparation of liposomal sanguinarine and study of its cytotoxic effect against prostate cancer cells. Russian nanotechnology. 2020;15(2):231–237.
12. Soloveva N. L., Sokurenko M. S., Zyryanov O. A. Bioavailability of curcumin and methods of its enhancement (review). Drug development & registration. 2018;(3):46–53. (In Russ.)
13. Zorina T. E., Yankovsky I. V., Yakovets I. V., Kravchenko I. E., Ermilova T. I., Shman T. V., Belevtsev M. V., Zorin V. P. Intracellular localization and phototoxicity mechanisms of chlorin E6 derivatives and their liposomal formulations. Biophysics. 2019;4:674–685. (In Russ.) DOI: 10.1134/S0006302919040057.
14. Valitova Ju. N., Renkova A. G., Minibayeva F. V. β-sitosterin – natural antioxidant. Ecobiotech. 2020;3(2):150–156. (In Russ.) DOI: 10.31163/2618-964X-2020-3-2-150-156.
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Molokhova E.I., Lipin D.E., Sorokina Yu.V., Luzhanin V.G. Development and evaluation of the quality of phytosomes with ecdysteroids Seratula coronata L. Drug development & registration. 2025;14(3):137-145. (In Russ.) https://doi.org/10.33380/2305-2066-2025-14-3-2072
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