Post-column derivatization in pharmaceutical analysis

Abstract

Post-column derivatization in HPLC is used to analyze low concentration of compounds that do not contain chromophore or fluorophore groups. In pharmaceutical analysis, this technique occupies a special niche and is used for the analysis of amino acids in protein and peptide drugs in accordance with SP RF, EP, USP as well as for determination of polyether monocarboxyl ionophore antibiotics (monensin, narasin, salinomycin, semduramicin, maduramicin, etc.) and others. The method is characterized by high repeatability due to the absence of matrix influence on the results of analysis and stability of the obtained derivatives, full automation of the process, as well as a fast and simple one-step derivatization reaction without the formation of by-products. Over than 12 years, «Sevko & Co» company has been offering the unique solution for Russian market – the ARM-1000H(T) post-column derivatization module, which is compatible and can be used with any HPLCs. The module can be equipped not only with a chemical but also with a photochemical reactor, expanding the range of its applications. The company has developed a turn-key solution for amino acid analysis – the AАA-1000 amino acid analyzer as a consequence and a result of continuous research into peculiarities of post-column derivatization technique. This chromatograph consists of standard modules of the «Skorohod» HPLC series and can be easily modified to solve any analytical problem requiring post-column derivatization. The solution includes all the necessary reagents and ion exchange columns of own production for amino acid analysis. The analyzer can also be used for conventional analyses using reversed-phase or normal-phase chromatography. The unique possibility of creating a second completely independent analytical channel based on the autosampler from the "Skorohod" series eliminates any risk of cross-contamination between different analyses. The analyzer is manufactured at the «Sevko and Co» own facilities in a full cycle without the use of Western components and is the only domestic product of this kind.