DEVELOPMENT AND VALIDATION OF BIOANALYTICAL METHOD FOR QUANTIFICATION OF PINOLINE AND ITS METABOLITE 6-OH-1,2,3,4-TETRAHYDROCARBOLINE FOR THE PURPOSE OF CYPD26 ACTIVITY DETERMINATION
Abstract
A sensitive method for quantification of pinoline and its metabolite in human plasma using solid phase extraction and high performance liquid chromatography with mass-spectrometry was developed. Analytical range was 250-1500 pg/ml both for pinoline and for 6-hydroxy-1,2,3,4-tetrahydro-β-carboline. The developed method may be used to determine the current status of CYP2D6 activity.
About the Authors
R. K. Abdrashitov
I.M. Sechenov First Moscow State Medical University
Russian Federation
Russian Federation
A. E. Petukhov
I.M. Sechenov First Moscow State Medical University;
National Research Center Institute of Immunology Federal Medical Biological Agency of Russia
Russian Federation
Russian Federation
V. V. Smirnov
I.M. Sechenov First Moscow State Medical University;
National Research Center Institute of Immunology Federal Medical Biological Agency of Russia
Russian Federation
Russian Federation
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Review
For citations:
Abdrashitov R.K., Petukhov A.E., Smirnov V.V. DEVELOPMENT AND VALIDATION OF BIOANALYTICAL METHOD FOR QUANTIFICATION OF PINOLINE AND ITS METABOLITE 6-OH-1,2,3,4-TETRAHYDROCARBOLINE FOR THE PURPOSE OF CYPD26 ACTIVITY DETERMINATION. Drug development & registration. 2016;(1):190-194. (In Russ.)
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