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INVESTIGATION OF AMINO ACID PROFILE ZINGIBER OFFICINALE (ZINGIBERACEAE) BY THE THIN LAYER CHROMATOGRAPHY AND SPECTROPHOTOMETRIC METHODS

Abstract

Extracts from rhizomes of ginger (Zingiber officinale) are widely used in the production of biologically active additives and in the food industry. In addition to essential oils and phenolic compounds, ginger is also rich in amino acids. To identify the amino acid profile, a qualitative and quantitative analysis of the rhizomes of ginger was carried out. As a result of the experiments, the following amino acids were identified by thin-layer chromatography: arginine, histidine, glutamic acid, isoleucine, valine, proline, leucine, methionine. By means of spectrophotometric analysis, the number of amino acids was determined to be 10.22%. The relative error of the single definition with a confidence probability of 95% is 1.89%. Thus, we can conclude that further research and improvement of approaches to standardization of rhizomes of ginger, as well as the creation of new dosage forms based on it.

About the Authors

N. I. Savitskiy
Saint-Petersburg State Chemical-Pharmaceutical Academy
Russian Federation


A. B. Legosteva
Saint-Petersburg State Chemical-Pharmaceutical Academy
Russian Federation


References

1. B. Tepe, M. Sokmen, H.A. Akpulat, A. Sokmen. Screening of the antioxidant potentials of six Salvia species from Turkey // Food Chemistry. 2006. V. 95(2). P. 200-204.

2. M. Yoshikawa, S. Hatakeyama, N. Chatani, Y. Nishino, J. Yamahara. Qualitative and quantitative analysis of bioactive principles in Zingiberis rhizoma by means of high performance liquid chromatography and gas liquid chromatography. On the evaluation of Zingiberis rhizoma and chemical change of constituents during Zingiberis rhizoma processing // Yakugaku Zasshi. 1993. V. 113(4). P. 307-315.

3. H. Bliddal, A. Rosetzsky, P. Schlichting et al. A randomized, placebo-controlled, cross-over study of ginger extracts and Ibuprofen in osteoarthritis // Osteoarthritis and Cartilage. 2000. V. 8(1). P. 9-12.

4. N. Mascolo, R. Jain, S.C. Jain, F. Capasso. Ethnopharmacologic investigation of ginger (Zingiber officinale) // Journal of Ethnopharmacology. 1989. V. 27(1-2). P. 129-140.

5. S. Phillips, R. Ruggier, S.E. Hutchinson. Forum. Zingiber officinale (Ginger) - an antiemetic for day case surgery // Anaesthesia. 1993. V. 48(8). P. 715-717.

6. U. Jana, R.N. Chattopadhyay, B.P. Shaw. Preliminary studies on anti-inflammatory activity of Zingiber officinale Rosc., Vitex negundo Linn. and Tinospora cordifolia (Willid) Miers in albino rats // Indian Journal of Pharmacology. 1999. V. 31(3). P. 232-233.

7. J.R. Okalebo, K.W. Gathua, P.L. Woomer. Laboratory Methods of Soil and Plant Analysis: A Working Manual. 2nd ed. - Nairobi, Kenya: TSBF-CIAT and SACRED Africa. 2002.

8. D. Bercovici, F. Fuller. Industrial amino acids in nonruminant animal nutrition // Biotechnology in animal feeds and animal feeding / Ed. by R.J. Wallace, A. Chesson. - Weinheim: VCH. 1995. P. 93-113.

9. A. Ambrogelly, S. Palioura, D. Söll. Natural expansion of the genetic code // Nat Chem Biol. 2007, V. 3. P. 29-35.

10. E.N. Marieb. Essentials of Human Anatomy and Physiology. 10th ed. - San Francisco: Benjamin Cummings, 2012.

11. О.Ю. Щепочкина, Н.Б. Демина, А.А. Жогова, М.Н. Анурова, О.Ю. Вальчихина, А. Надер. Определение биологически активных веществ в сухом экстракте имбиря лекарственного (Zingiber officinale Roscoe) // Разработка и регистрация лекарственных средств. 2015. № 2(11). С. 160-164.

12. О.Ю. Вальчихина, А. Надер, Н.Б. Демина. Корневище имбиря как перспективное растительное сырье для создания лекарственных средств // Разработка и регистрация лекарственных средств. 2015. № 4(13). С. 82-90.


Review

For citations:


Savitskiy N.I., Legosteva A.B. INVESTIGATION OF AMINO ACID PROFILE ZINGIBER OFFICINALE (ZINGIBERACEAE) BY THE THIN LAYER CHROMATOGRAPHY AND SPECTROPHOTOMETRIC METHODS. Drug development & registration. 2017;(2):208-212. (In Russ.)

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ISSN 2305-2066 (Print)
ISSN 2658-5049 (Online)