POLYEXTRACTION OF HERBS OF ECHINACEA BY SYSTEMS OF EXTRACENTS WITH RISING POLARITY

The process of extracting the Echinacea herbs (Echinacea Moench) has been developed with a series of water-alcohol solutions with an alcohol concentration of 70%, 40%, 20%. Extraction was carried out in the vacuum boiling mode with periodic pulsations of the vacuum. Such a regime provided high speed of the process and the possibility of extracting both lipophilic (chlorophyll derivatives, carotenoids) and hydrophilic (chicory acid and its derivatives) BAS. The kinetics of BAS extraction at three stages of maceration was studied: chicory acid is extracted by the polar extractant at all stages of extracting; the lipophilic components pass into the extraction at the stage of treatment with an alcoholconcentration of 70%. Method of micropreparative thin-layer chromatography with eluting and UV-analysis of the eluates was used to determine the groups of extractable BAS. A comparison of dry extracts obtained by water extraction, 24% alcohol extraction and extraction of a number of extractants with sequentially increasing polarity was made. It is established that the method of polyextraction allows to obtain a product containing the widest spectrum of BAS Echinacea.

natural immunomodulators, herb echinacea, extraction, UV spectroscopy, TLC, chicory acid, echinacoside

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