STUDY OF QUALITY INDICATORS AND TECHNOLOGICAL CHARACTERISTICS OF LEAVES, STEMS AND FLOWERS OF INULA VISCOSA (L.) AS PROMISING RAW MATERIALS

The article contains information about the fragment of the complex study on the introduction of Inula viscosa L. to the pharmaceutical practice. Inula viscosa L. is a plant growing in Syria. Its aqueous extraction and leaf powder is used in the Mediterranean traditional medicine for the treatment of dermatological pathologies. Based on the published scientific data on antimicrobial activity and the identified prospects for the production and use of preparations based on Inula viscosa L., a pharmacological and pharmacognostic study of leaf and herb raw materials is carried out using modern pharmacopoeial methods of research and standardization. The results of research of some pharmacognostic characteristics of new types of raw materials are presented for Inula viscosa L. leaves and herb. Quality indicators of raw materials are established: moisture content in leaves is not more than 5%, amount of herb is not more than 8%, ash content is not more than 11% in leaves and herb and ash, insoluble in 10% solution of hydrochloric acid is not more than 1% in both types of raw materials, content of substances extracted by water is about 32%. The technological characteristics of the crushed raw materials are studied: bulk density, bulk mass, absorption coefficients of solvents with different ethanol content, based on the values of which 70% ethanol is selected as extractant for extraction. 70% ethanol is able to extract a multicomponent target complex of substances including phenolic acids, flavonoids, tannins, sesquiterpenoids. The absorption coefficients of this extractant are 1,15 ml/g for herb and 1,22 ml/g for leaves. TLC is proposed for the identification of the plant raw materials and for the detection of flavonoids and phenolcarbonic acids (caffeic and chlorogenic acids) in aqueous and alcoholic extracts. There were identified two maxima of the absorption in the UV spectrum of the alcoholic extraction of Inula viscosa L. at wavelength 298 and 329 nm. It allows to assume the presence of phenolcarbonic acids and other phenolic substances including tanning substances and flavonoids in the extraction. The obtained absorption spectrum can serve as a specific characteristic for identification of medicinal plant material and extraction of Inula viscosa L. To develop the technology of extracts, the fractional composition of the Inula viscosa L. crushed raw material is determined. The majority of particles (about 44%) in the crushed herb of Inula viscosa L. have the size more than 2 mm, while the crushed leaves represent the fraction with majority of particles (about 45%) having size less than 2 mm but more than 1 mm. The obtained data is supposed to be used for the development of the draft normative documentation for Inula viscosa L. raw materials and for the development of technology for obtaining extraction preparations based on it.

Inula viscosa (L.), medicinal plant raw materials, technological characteristics, phenol carboxylic acids, flavonoids

1. State Pharmacopoeia, XIII ed. Т. 1. М., 2015. 1470 р.

2. Mussa R., Shineva N. V., Suslina S. N. Pharmacognostic characteristics of raw materials and the target fragment of the metabolite Inula viscosa // The fifth scientific and practical conference with international participation: «Young Scientists and Pharmacy of the XXI Century» / Collection of scientific papers. M., VILAR, 2017. Р. 93-99.

3. Fontana G., La Rocca S., Passannanti S. Sesquiterpene compounds from Inula viscosa // Natural Product Research. 2007. № 4. P. 124-127.

4. Parolin P., Ion Scotta M., Bresch C. Notes on the phenology of Dittrichia viscosa // Journal of Mediterranean Ecology. 2013. V. 12. P. 27-35

5. Máñez S., Hernández V., Giner R. M. Inhibition of pro-inflammatory enzymes by inuviscolide, a sesquiterpene lactone from Inula viscosa // Fitoterapia. 2007. № 78. P. 329-331.

6. Brullo S., De Marco G. Taxonomical revision of genus Dittrichia (Asteraceae) // Portugaliae Acta Biol. 2000. № 19. P. 341-354.

7. Amin S., Kaloo Z. A., Singh S., Altaf T. Medicinal importance of genus Inula- a review // Int. J. Cur. Res. Rev. 2013. № 5. P. 20-26.