DEVELOPMENT AND VALIDATION OF THE METHOD OF VITAMINE E DETERMINATION IN MICROEMULSION COMPOSITIONS BY HIGH-EFFECTIVE LIQUID CHROMATOGRAPHY
Abstract
The results of development and validation of the method of vitamin E detrmination in microemulsion compositions suitable for its controlling in transdermal delivery systems and also for the incoming control of tocopherol acetate oil solutions presents in the article. The method is based on the vitamin E extraction from the microemulsion by a mixture of acetonitrile - ethyl acetate (80:20) and subsequent analysis by reversed-phase HPLC. The main validation characteristics of the method meet the eligibility criterias. The analytical area of the methodology was from 0.1 to 10.0 mg/g vitamin E concentration. The results of method approbation on transdermal delivery system laboratory samples on microemulsions based are presented.
About the Authors
S. V. Kursakov
Academician V. I. Shumakov National Medical Research Center of Transplantology and Artificial Organs, Ministry of Health of the Russian Federation;
ANO «Institute of biomedical research and technology»
Russian Federation
Russian Federation
E. G. Kuznetsova
Academician V. I. Shumakov National Medical Research Center of Transplantology and Artificial Organs, Ministry of Health of the Russian Federation
Russian Federation
Russian Federation
O. M. Kuryleva
Academician V. I. Shumakov National Medical Research Center of Transplantology and Artificial Organs, Ministry of Health of the Russian Federation
Russian Federation
Russian Federation
L. A. Salomatina
Academician V. I. Shumakov National Medical Research Center of Transplantology and Artificial Organs, Ministry of Health of the Russian Federation
Russian Federation
Russian Federation
V. I. Sevastianov
Academician V. I. Shumakov National Medical Research Center of Transplantology and Artificial Organs, Ministry of Health of the Russian Federation
Russian Federation
Russian Federation
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Review
For citations:
Kursakov S.V., Kuznetsova E.G., Kuryleva O.M., Salomatina L.A., Sevastianov V.I. DEVELOPMENT AND VALIDATION OF THE METHOD OF VITAMINE E DETERMINATION IN MICROEMULSION COMPOSITIONS BY HIGH-EFFECTIVE LIQUID CHROMATOGRAPHY. Drug development & registration. 2018;(3):88-95. (In Russ.)
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