OPTIMIZATION OF HPLC METHODS FOR QUANTITATIVE DETERMINATION OF GLYCYRRHIZIC ACID IN LICORICE ROOTS
Abstract
Comparative evaluation of glycyrrhizic acid content in licorice roots by methods of UV-spectrophotometry (GF RF XIII) and high-performance liquid chromatography (HPLC) was carried out. Sample preparation for HPLC method of quantitative determination of glycyrrhizic acid in samples of domestic production is developed. Small selectivity of UV-spectrophotometry at determination of glycyrrhizic acid is experimentally established and perspective of HPLC method for standardization of licorice raw materials is shown.
About the Authors
B. V. Brovchenko
LLC «AZT PHARMA K.B.»
Russian Federation
Russian Federation
V. A. Ermakova
Sechenov First State Medical University
Russian Federation
Russian Federation
A. N. Kuzmenko
Sechenov First State Medical University
Russian Federation
Russian Federation
I. A. Samylina
Sechenov First State Medical University
Russian Federation
Russian Federation
I. I. Krasnyuk (Jr.)
Sechenov First State Medical University
Russian Federation
Russian Federation
References
1. ФС 2.5.0040.15. Солодки корни // Государственная фармакопея РФ. XIII изд. - М., 2015.
2. ФС 573. Корень солодки // Государственная фармакопея СССР. Х изд. - М.: Медицина, 1968.
3. The United States Pharmacopeia, USP30 NF25. 2009. V. 2. Liquorice. P. 2263.
4. European Pharmacopoeia 7th edition: Liquorice root - Liquiritiae radix 01/2011: 0277.
5. Pharmacopoeia of the people’s republic of China. 2005. V. 1. P. 207-2009.
6. The Japanese Pharmacopoeia, 14th ed. Ministry of Health, Labour and Welfare, Tokyo, Japan Part 2. 2002. P. 932-933.
7. Gavrilin M. V., Senchenko S. P., Tamiryan A. M., Pechenova A. V. Perfection of methods for assessing the quality of roots and licorice syrup // Chemistry of plant raw materials. 2009. № 4. P. 147-150.
8. Hennell J. R., Lee S., Khoo C. S. The determination of glycyrrhizic acid in Glycyrrhiza uralensis Fisch. ex DC. (Zhi Gan Cao) root and the dried aqueous extract by LC-DAD // J. Pharm. Biomed. Anal. 2008. V. 47. P. 494-500.
9. Stolyarova O. V., Baltina L. A., Mikhailova L. R. etc. Optimization of the method for obtaining the monoammonium salt of glycyrrhizic acid from the roots of the licorice of the Ural Glycyrrhiza uralensis Fisch.) Of Siberian populations // Chemistry for Sustainable Development. 2008. V. 16. № 5. P. 571-576.
10. Wang P., Li S. F. Y., Lee H. K. Simultaneous analysis of glycyrrhizin, paeoniflorin, quercetin, ferulic acid, liquiritin, formononetin, benzoic acid and isoliquiritigenin in the Chinese proprietary medicine Xiao Yao Wan by HPLC // J. Chromatograph. A. 1998. V. 811. № 1-2. P. 219-224.
11. Kondratenko R. M., Baltina L. A., Mikhailova L. R., Danilov V. T., Gabbasov, T. M., Murinov Y.I., Tolstikov G.A. The preparation of glycyrrhizic acid and its practically important salts from the extract of licorice root // Khimiko-pharmaceutical magazine. 2005. V. 39. № 2. P. 33.
Review
For citations:
Brovchenko B.V., Ermakova V.A., Kuzmenko A.N., Samylina I.A., Krasnyuk (Jr.) I.I. OPTIMIZATION OF HPLC METHODS FOR QUANTITATIVE DETERMINATION OF GLYCYRRHIZIC ACID IN LICORICE ROOTS. Drug development & registration. 2018;(3):162-165. (In Russ.)
Views:
1260
Email this article 