Features of Microelements Express-determination in Medicinal and Nonoficinal Plants by X-Ray-Fluorescence Analysis

Introduction. The chemical composition of plants depends on the elemental composition of the habitat, and also reflects the species and individual characteristics of the plant. Before using any plant for medicinal purposes, it is also necessary to collect as much information as possible about the elemental composition of the feedstock.

Aim. Consequently, it became possible to create a method for the rapid determination of trace elements in plants using the X-Ray fluorescent method.

Materials and methods. In accordance with the international intercalibration standard in the IAEA system (IAEA-433), an international standard sample NIST SRM 2976, certified by the National Institute of Standards and Technology (USA), was used to provide external quality control (QA/QC) of laboratory elemental analysis. For Zn the results of plant raw powder materials analysis by XRF (express analysis) were compared with AAS by electrothermal atomization and Zeeman background correction after microwave acid mineralizationof biomaterials. To ensure the reproducibility of the results of XRF-express analysis, it is absolutely necessary to carry out mild drying and dispersion of the plant material.

Results and discussion. The results of X-ray fluorescence (XRF) analysis of the elemental composition of the fruits (fructus Anethi graveolens L.), the seeds (semina Cucurbitae pepo L., semina Menthae arvensis L., semina Cucumis sativa L., semina Kalanchoës daigremontianae) and leaves (folia Callisiae fragrantis L.) of medicinal and nonoficinal plants are presented in the article. The methods of XRF and AAS analysis have been developed for the technology of essential micoelements enrichment in of Callisiae fragrantis control leaves at different ratios of hydrogen isotopologues (D/H) in the water.

Conclusion. For example, zinc(II) has demonstrated the ability to create modified plants with high content of essential microelement, up to 1.4 mg /g dry weight.

1. Vernandskiy V. I. The Biosphere and noosphere. – Moscow: Science. 1989. (In Russ.).

2. Syroeshkin A. V., Matveeva I. S., Chikviladze G. N. Results of longterm intercomparison exercises in IAEA system. Trace Elements in Medicine. 2010; 11(1): 11–14 (in Russ.)

3. Krasovsksii P. A., Karpov O. V., Balkhanov D. M., Lesnikov E. V., Darkin D. A., Ulyantsev A. S., Matveeva I. S., Pleteneva T. V., Syroeshkin A. V. Nanoparticales in natural mineral waters. Measurement procedure and results. Measurement Techniques. 2010; 53(8): 852–857. https://doi.org/10.1007/s11018-010-9587-6

4. Calder J. A., Jamieson W. D. The Purpose and Program of the IOC/UNEP/IAEA Group of Experts on Standards and Reference Materials. Fresenius Journal of Analytical Chemistry. 1990; 338: 378–379. https://doi.org/10.1007/bf00322497.

5. Balyshev A. V., Grebennikova T. V., Syroeshkin A. V. The substantiation of antituberculous effect of the new Zn²⁺-contained pharmaceutical (microelement profiles, cell immunity, nonspecific resistance). Trace Elements in Medicine. 2004; 5(4): 6–8 (in Russ.).

6. Goncharuk V. V., Syroeshkin A. V., Zlatskiy I. A., Uspenskaya E. V., Orekhova A. V., Levitskaya O. V., Dobrovolskiy V. I., Pleteneva T. V. Quasichemical description of the cell death kinetics of cellular biosensor Spirostomum Ambigua for testing the biological activity of aqueous solutions. Journal of Water Chemistry and Technology. 2017; 39(2): 97–102.