THE DEVELOPMENT OF A PRODUCTION SCALE TECHNOLOGY OF RECOMBINANT FOLLICLE STIMULATING HORMONE BIOPROCESS IN CHO CELL LINE
Abstract
The article discusses the development of a production technique for recombinant follicle-stimulating hormone used in the treatment of infertility, in the culture of Chinese hamster ovary cells. As part of the creation of the technological platform receiving hormone the bioprocess in wave-induced motion bioreactor has been optimized. A number of parameters that influence on the bioprocess was studied, as follows: optimal cell density, concentration of metabolites, temperature shift and its duration.
About the Authors
D. A. Gusarov
Research-Educational Internet Portal «ExpertBIOTECH»
Russian Federation
Russian Federation
A. I. Gosudarev
Experimental Biotech Facilities, N.F. Gamaleya Research Center of Epydemiology and Microbiology
Russian Federation
Russian Federation
V. D. Gusarova
Experimental Biotech Facilities, N.F. Gamaleya Research Center of Epydemiology and Microbiology
Russian Federation
Russian Federation
D. V. Byrikhina
Experimental Biotech Facilities, N.F. Gamaleya Research Center of Epydemiology and Microbiology
Russian Federation
Russian Federation
F. M. Izhaeva
Experimental Biotech Facilities, N.F. Gamaleya Research Center of Epydemiology and Microbiology
Russian Federation
Russian Federation
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Review
For citations:
Gusarov D.A., Gosudarev A.I., Gusarova V.D., Byrikhina D.V., Izhaeva F.M. THE DEVELOPMENT OF A PRODUCTION SCALE TECHNOLOGY OF RECOMBINANT FOLLICLE STIMULATING HORMONE BIOPROCESS IN CHO CELL LINE. Drug development & registration. 2016;(2):80-84. (In Russ.)
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