Application of a Charged Aerosol Detector (CAD, Thermo Fisher Scientific) for the Analysis of Biopharmaceuticals

Abstract

The present work is devoted to the problems of identifying critical components and excipients of biopharmaceuticals, such as O-linked glycans released from glycoproteins by reductive в-elimination; sialic acids; and vaccine adjuvants. The main problem of HPLC analysis of such compounds is the absence of a detection system that is independent of the presence or absence of chromophore and fluorophore groups in the molecule and gives an almost universal response regardless of the chemical structure of the analyte. The article describes the characteristics of a charged aerosol detector (CAD), the use of which makes it possible to solve most of the problems associated with the detection of chromophore-free substances. The article demonstrates several examples where CAD can be used to detect and quantify substances separated by reverse phase chromatography and hydrophilic interaction chromatography.

The article is presented by the company LLC ''AVRORA''