Simultaneous Analysis of Combination Active Pharmaceutical Ingredients by HLPC SCION LC 6000 with Diode Array Detector

Abstract

An important problem for pharmaceutical and toxicological laboratories is quick and accurate determination of mixtures of active pharmaceutical ingredients in various samples, including blood samples. This article shows the simultaneous quantitative analysis and identification of two common pharmaceutical ingredients (paracetamol and ibuprofen) on a SCION LC 6000 liquid chromatograph with a diode array detector. As a result of the experiment, it was shown that with the help of a diode-array detector with multi-wavelength function, it is possible to determine both substances in one injection. Excellent linearity and repeatability of the SCION HPLC-DAD was demonstrated with low RSD values and high R² values.